The invention provides an inclined angle flexible needle robot auxiliary puncture system based on piezoelectric actuation, relates to robot auxiliary puncture systems, and aims at resolving the problems that an existing flexible needle is complex in stress, brings difficulty to trajectory planning, and does not easily guarantee moving accuracy in the moving process inside tissue. According to the inclined angle flexible needle robot auxiliary puncture system, a support piece, a lead screw tail end bearing block, a lead screw head end bearing block and a motor installation base are sequentially arranged on a platform from left to right, an inserting needle motor is arranged on the motor installation base, an output shaft of the inserting needle motor is connected with a lead screw, a guide rail is arranged on the platform, a moving nut is arranged on the lead screw, a bearing support seat is arranged on the moving nut, a sliding block is arranged on the guide rail, the bearing support seat is connected with the guide rail, a sliding block nut connecting seat is arranged on a sliding block nut connecting piece, a flexible needle fixing piece is fixedly installed on the sliding block nut connecting seat, a support sleeve is arranged at the upper end of the support piece, one end of a feeding flexible needle is arranged inside the support sleeve in a penetrated mode, and the other end of the feeding flexible needle is connected with the flexible needle fixing piece. The inclined angle flexible needle robot auxiliary puncture system is applied to robot auxiliary puncturing.