The application provides a focusing
system and a focusing method. The focusing
system comprises a multi-channel illumination unit, a projection
grating mask, a light splitting
prism, an
imaging lens, a light splitting plate, a
microscope objective, a detection
grating mask, a converging lens, a
detector and an object table for carrying a sample to be measured, and further comprises a displacement driving device. The focusing
system comprises first, second and third optical axes. The first and third optical axes are parallel, and the first and second optical axes are perpendicular. The first
optical axis is a common central axis of the sequentially arranged multi-channel illumination unit, the projection
grating mask and the light splitting
prism. The second
optical axis is a common central axis of the sequentially arranged light splitting plate, the
imaging lens, the light splitting
prism, the detection grating mask, the converging lens and the
detector. The third
optical axis is a common central axis of the sequentially arranged light splitting plate and the
microscope objective. The displacement driving device is used for adjusting the axial relative position of the
imaging lens on the second optical axis. The application reduces the defocus
signal detection error and improves the imaging
clarity.