The invention provides a non-cooperative interference cancellation device and method for partial frequency band protection. The device comprises a microwave delay line, a synthesizer, a coupler, N auxiliary antennas, N + 1 down converters, N + 1 analog-to-digital converters, a digital signal processing unit, a digital-to-analog converter and an up converter, N is greater than or equal to 1, interference cancellation processing is carried out in an analog domain, and adaptive beam forming is carried out in a digital domain, and anti-interference processing of partial frequency points in a wide working frequency band is realized. The method provided by the invention can be used for interference protection of part of narrowband frequency points in a wide working band without influencing other signal frequency points which are not interfered, can avoid the problem of high-speed sampling of broadband signals, reduces the requirement on the sampling rate of a digital-to-analog converter, and greatly improves the receiving signal-to-noise ratio index; and beam forming signal processing can be realized in a digital domain under the conditions of a complex multi-path environment and a large number of antennas, so that the anti-interference performance is improved.