The invention relates to the field of kits, specifically a DNA extraction kit, comprising a box body, a rotating structure, a fixed structure, a clamping structure, a freezing structure, a pulling structure and a moving structure; The structure can not only play a role of sealing protection for the box body, but also ensure that the test tubes placed inside the box body are always in a vertical state, so as to avoid the leakage of the test tube when the box body is transported, which greatly increases the reagent consumption. Excellent transportation safety, and with the freezing structure installed inside the box, it can not only refrigerate the reagents placed inside the box, but also collect and process the condensed water droplets outside, and can also achieve rapid installation And disassembly, easy to operate, by setting a fixed structure inside the rotating structure where the test tube is placed, the outer side wall of the inserted test tube can be locked and fixed, avoiding the shaking of the test tube when transporting reagents, and preventing liquid leakage.