The invention discloses a lactation promoting material composition containing phosphatidylserine, lactation promoting soup containing phosphatidylserine, and a making method of the lactation promoting soup. The composition comprises water, phosphatidylserine, radix angelicae sinensis, rhizoma ligustici wallichii, white peony roots, radix rehmanniae, dishcloth gourd kernels, papayas, peanuts, red jujubes and crystal sugar, wherein based on 1000 parts by weight of the water, 1-5 parts by weight of the phosphatidylserine, 10-30 parts by weight of the radix angelicae sinensis, 10-30 parts by weight of the rhizoma ligustici wallichii, 10-30 parts by weight of the white peony roots, 20-50 parts by weight of the radix rehmanniae, 40-80 parts by weight of the dishcloth gourd kernels, 100-200 parts by weight of the papayas, 40-100 parts by weight of peanuts, 30-50 parts by weight of the red jujubes, and 80-200 parts by weight of the crystal sugar are added. The effects that lactation can be effectively promoted, the phosphatidylserine can be complemented, and good nutrient components are provided for postpartum lying-in women are achieved.