The invention relates to the technical field of pepper seedling cultivation, and discloses a cultivation method of overwintering pepper seedlings. The cultivation method of the overwintering pepper seedlings comprises the following steps of S1, selecting seeds and soaking the seeds in pesticide; S2, controlling the temperature to germinate; S3, carrying out base-divided seedling raising; S4, carrying out field management; and S5, transplanting seedlings. According to the method, a warm water and liquid medicine soaking method is adopted in the seed selection stage, the seeds soaked with a sodium phosphate solution and a sodium hydroxide solution can effectively prevent virus diseases, the seeds soaked with the copper sulfate can effectively prevent anthracnose and bacteria, the disease resistance of the seeds is enhanced, and germination of the seeds can be effectively promoted through temperature control germination, so that the germination time of the seeds is effectively prolonged,the subsequent seedling strengthening rate is high, and it is guaranteed that the seedlings can adapt to environment changes and cannot die due to temperature changes; and by adopting a ridged planting mode, the fertilization amount and the pesticide spraying amount can be effectively controlled, and the situation that due to uneven fertilization or pesticide spraying, the seedling growth difference is too large, consequently the subsequent fruiting rate is uneven, and the yield is lowered is avoided.