The invention provides a preparation method of an
exosome as well as the
exosome prepared by adopting the method and applications of the
exosome. The method comprises the following steps: (1) collecting the human
adipose tissue, and carrying out extracting to obtain adipose-
derived stem cells; and (2) carrying out treatment on the adipose-
derived stem cells obtained in step (1), and thus the exosome is obtained through extraction. The exosome is the exosome derived from the adipose-
derived stem cells, when the exosome is used for
skin repair, the
wound healing speed can be accelerated, the pain of patients can be alleviated, the wound
recovery quality can be improved, and the area of
scars can be reduced. The average time for the
wound healing of the untreated group is 33.7 days, after thegel preparation containing the exosome provided by the invention is applied clinically, the
wound healing only needs 12.2 days, thus the pain of the patients of is obviously alleviated, and the woundinfection probability is reduced; the effect for the
refractory wound such as diabetes is also very obvious, the effective rate achieves 85.7%, but the effective rate of the control group is only 42.1%.