The invention relates to a process for domesticating gradient dynamic active sludge under an aerobic condition. The process comprises the following steps: (1) preparing simulated wastewater as a culture medium for later use according to a mass ratio of 100:75:5:1 of BOD5:C:N:P; (2) adding the active sludge and the culture medium obtained in the step (1) into an SBR (styrene butadiene rubber) reaction tank with an effective volume of 15L, stirring for aeration, and changing water once every 12 hours under the aerobic condition, namely each sludge age is 12 hours; changing 3L of water each time and controlling the DO value to be 1.5mg / L from the first day to the sixth day; changing 4.5L of water each time and controlling the DO value to be 2.0mg / L from the seventh day to the twelfth day; and changing 6L of water each time and controlling the DO value to be 2.5mg / L from the thirteenth day to the eighteenth day. According to the process provided by the invention, the culture medium is added in a gradient manner during domestication, the add amount is different in different periods, and the gradient is increased, so that the microbial quantity can be increased as much as possible; and by adopting the process provided by the invention, after 18 days of culture domestication, the color of the sludge is changed from black into reddish brown, the sludge has obvious fishy smell, and the sedimentation performance of the sludge is obviously improved.