The present invention specifically discloses a diamondback moth larva pure artificial feed and a preparation method thereof. The feed contains malt powder, casein, sucrose, a multivitamin mixture, cellulose, agar and other auxiliary materials, and is determined through comparison and optimization. According to the present invention, the feed contains nutrients required by health growth of diamondback moth larva, and the ratio of various components is within a normal range; the feed is not added with vegetable leaf powder components, such that no pesticide residue in the feed is ensured, and the fed diamondback moth is the pure line sensitive species; and with the feed, a lot of diamondback moth polypides with characteristics of uniform development and the same instar can be obtained, and scientific research requirements can be completely met.