The invention discloses thermosensitive color-changing microcapsule UV (Ultraviolet) photo-cured printing ink and a preparation method thereof, and belongs to the technical field of printing ink. The thermosensitive color-changing microcapsule UV photo-cured printing ink is prepared from the following raw materials in parts by weight: 22 to 60 parts of epoxy acrylate, 15 to 25 parts of color developing microcapsule, 15 to 25 parts of polyester acrylate, 10 to 15 parts of dipropylene glycol diacrylate, 5 to 10 parts of trimethylolpropane triacrylate, 5 to 10 parts of acrylic acid-4-polyhydroxybutyrate, 0.5 to 3 parts of benzil dimethyl ketal, 1 to 5 parts of dispersing agent, 1 to 5 parts of hollow glass beads and 1 to 3 parts of UV varnish. The thermosensitive color-changing microcapsule UV photo-cured printing ink is cured by UV, and has the advantages of high drying speed, good fixing effect, clear meshes, water resistance, solvent resistance, abrasion resistance and the like, is almost zero in VOCs (Volatile Organic Compounds) emission, is environment-friendly, and has a wide application range and a higher application value.