The invention relates to a transformer substation monitoring system debugging method based on digital twinning, and the method employs a transformer substation monitoring system which comprises a station control layer, a spacing layer and a process layer. Comprising the following steps: S1, collecting configuration data of a running transformer substation; S2, realizing offline configuration and debugging work of an online system; S3, restoring and backing up the transformer substation on site; and S4, displaying static information and dynamic real-time running information of the transformer substation. The system has the advantages that the debugging interval of the transformer substation can be operated and visualized through the 3D holographic image information display system, a three-dimensional live-action model can be formed, after the digital twinning monitoring background is subjected to debugging change, the digital twinning monitoring background can automatically correspond to the change of the three-dimensional model, and the debugging efficiency of the transformer substation is improved. Defects such as abnormal configuration of a field operation transformer substation database can be synchronously judged through the digital twinborn monitoring background, the number of times of field repeated defect processing by maintainers in the past is reduced, and more than 30% of human resources can be saved.