The invention relates to the field of clamping equipment, and specifically discloses a manual positioning and clamping device, which includes a workbench, a cam handle, a first connecting rod, a second connecting rod, a slider, a floating block and claws. By rotating the cam handle, Make the first connecting rod move along the first horizontal direction, and drive the second connecting rod to rotate relative to the first connecting rod and the corresponding slider, and then use the second connecting rod to push the slider to move along the second horizontal direction, and the slider will Drive the corresponding floating block to rotate, so that the corresponding claws rotate around their own hinge shafts, so that the second ends of the two claws approach or move away from each other, so as to clamp or loosen the workpiece on the cover plate. The manual positioning and clamping device provided by the present invention has a simple structure. After the workpiece is placed on the workbench, it only needs to turn the cam handle to drive the first connecting rod, the second connecting rod, the slider and the floating block to move, and then the jaws can be used to realize The clamping and loosening of the workpiece, the clamping method is simple, the operation is quick, and the clamping efficiency is improved.