44 results about "Luminescent Assays" patented technology

Assay kits for increasing the sensitivity of luminescent assays are provided that include an organic compound that reduces luminescence that is not dependent on the presence of an analyte by at least about 10 fold, and that reduces, maintains, or increases the luminescence that is dependent on the presence of an analyte.

The present invention provides protein fragment complementation assays for drug discovery, in particular to identify compounds that activate or inhibit cellular pathways. Based on the selection of an interacting protein pair combined with an appropriate PCA reporter, the assays may be run in high-throughput or high-content mode and may be used in automated screening of libraries of compounds. The interacting pair may be selected by cDNA library screening; by gene-by-gene interaction mapping; or by prior knowledge of a pathway. Fluorescent and luminescent assays can be constructed using the methods provided herein. The selection of suitable PCA reporters for high-throughput or high-content (high-context) assay formats is described for a diversity of reporters, with particular detail provided for examples of monomeric enzymes and fluorescent proteins. Methods are described for constructing such assays for one or more steps in a biochemical pathway; testing the effects of compounds from combinatorial, natural product, peptide, antibody, nucleic acid or other diverse libraries on the protein or pathway(s) of interest; and using the results of the screening to identify specific compounds that activate or inhibit the protein or pathway(s) of interest. Single-color and multi-color assays are disclosed. Further disclosed are universal expression vectors with cassettes that allow the rapid construction of assays for a large and diverse number of gene / reporter combinations. The development of such assays is shown to be straightforward, providing for a broad, flexible and biologically relevant platform for drug discovery.

The invention relates to a stress luminescence measurement device and a stress luminescence measurement method. The stress luminescence measurement device according to a first aspect is provided with a load application mechanism configured to deform a sample (1) by applying a load to the sample (1), a light source (31) configured to emit excitation light to a stress luminescent object (2) arranged on a surface of the sample (1), a camera (40) configured to image luminescence of the stress luminescent object (2), and a controller (50) configured to control the load application mechanism, the light source (31), and the camera (40). The controller (50) acquires a deformation state of the sample (1) at the imaging timing by the camera (40) and stores the acquired deformation state of the sample (1) in association with the image captured by the camera (40) in a memory (502).