A machining method of a turbine rotor comprises the following processes of step a, performing rough turning on a pressure air end, a turbine disk and a turbine end of a turbine shaft; step b, performing drilling and heat treatment on a lifting hole; step c, performing heat treatment on the turbine shaft; step d, performing half-finished turning on the pressure air end, the turbine disk and the turbine end of the turbine shaft; step e, grinding outer circles and an end surfaces; step f, aligning the ground outer circles and end surfaces and performing finish turning on the pressure air end, the turbine disk and the turbine end of the turbine shaft; step g, grinding a tongue groove positioning circle and a key groove positioning circle; step h, aligning the tongue groove positioning circle and broaching a tongue groove; step i, aligning the key groove positioning circle and milling a key groove, an air guide wheel key groove and a pressure air impeller key groove; step j, performing finish turning an air sealing groove, an oil sealing groove and a center hole; step k, grinding stages of outer circles and end surfaces; step l, performing rounding on the key groove and performing deburring and chamfering on the tongue groove; step m, installing an air sealing ring on the turbine shaft; step n, performing finish turning on the end surface of the total length of the pressure air end and performing smooth transfer and polishing on the outer circles; step o, performing finish turning on the air sealing ring and performing smooth transfer and polishing on the outer circles; step p, performing appearance inspection. According to the machining method of the turbine rotor, the rotary accuracy of the turbine rotor is improved.