25 results about "Proteins metabolism" patented technology

The invention relates to the technical field of medical health management, in particular to a chronic kidney disease and diabetes collaborative nutrition monitoring and early warning method and system.The method comprises the steps that multi-dimensional physiological indexes, nutrition intake and sleep data of a patient are obtained, and a dynamically-coupled metabolic state topological space is constructed; based on the space, constructing a dual-disease metabolism coupling matrix, analyzing stability characteristics of a metabolism state trajectory, identifying stable and unstable attractors, and calculating a multi-dimensional risk score; a sleep-metabolism synchronism risk prediction model is introduced, and a personalized early warning threshold value is dynamically adjusted; when the risk score exceeds a threshold value, early warning is triggered, and intervention suggestions are generated, the interactive influence of protein metabolism and glycometabolism is quantitatively described from the metabolic mechanism level, and the contradiction of chronic kidney disease and diabetic patients in nutrition management is solved; limitation of a static threshold is broken through, and dynamic risk assessment is realized; sleep factors are creatively integrated, and a new way for improving the metabolic state is provided.

The invention belongs to the technical field of biological medicines, and particularly relates to application of poecilobdella manillensis in preparation of a medicine for treating renal injury. The invention provides a new application of poecilobdella manillensis in preparation of a medicine for treating renal injury, serum biochemical index detection proves that the poecilobdella manillensis can effectively reduce serum creatinine and blood urea nitrogen levels, increase serum albumin and total protein content, remarkably improve glomerular filtration function and protein metabolism function, and improve renal function. And the improvement degree of the traditional Chinese medicine composition is superior to that of a traditional Chinese medicine contrast. Although the curative effect is slightly poorer than that of a chemical drug group, the safety advantage is remarkable. The poecilobdella manillensis can comprehensively regulate renal function metabolism indexes, relieve kidney injury and avoid common side effects of chemical drugs, provides a new choice for clinical treatment of kidney injury, and is especially suitable for chronic kidney disease patients needing long-term treatment.

The present application provides methods and systems for single-run or minimally sequential detection and quantification of proteins, metabolites, and lipids using a unified instrumentation setup spanning a broad dynamic range (˜1 ng / L to 100 mg / L). In some embodiments, a two-phase database architecture transitions newly observed analytes from a discovery repository into a validated repository, enabling reproducible detection (CV<10%) with precise parameters (e.g., retention time, transitions). A machine-learning pipeline enhances automated peak selection by integrating large-scale manual curation with advanced feature extraction. The disclosed platform supports high-throughput multi-omics profiling of plasma or dried blood spot (DBS) samples and enables correlation with clinical factors such as age, BMI, or genetics. These systems maintain high sensitivity, scalability, and reproducibility, addressing long-standing limitations in clinical proteomics. As a result, the disclosed approach facilitates translational research, remote patient monitoring, and global healthcare implementation.

The present disclosure can improve the degree of soy protein proteolysis and the ability to produce valine, isoleucine, and leucine, which are branched-chain amino acids, as well as other amino acids, such as threonine, glycine, tyrosine, and lysine. Accordingly, the present disclosure can prevent sarcopenia and has antioxidant activity. Also, the present disclosure can maintain protein metabolic balance and relieve sarcopenia by muscle synthesis.The self-aggregation, hydrophobicity, and intestinal adhesion of a mixed strain is improved compared to a case where a single strain used for the mixed strain is used. Therefore, when the mixed strain of the present disclosure is ingested, the strain can remain for a long time in the intestine.

The invention provides a milk flavor essence and a preparation method thereof. The method comprises the following steps: preparing an activated bacterium solution from German-type lactobacillus bulgaricus subspecies; anhydrous cream and whey powder are prepared into a fermentation substrate; adding the activated bacterial liquid into the fermentation substrate, and performing sealed fermentation to obtain a fermentation product; and carrying out first sterilization treatment on the fermentation product, carrying out centrifugal separation, and taking supernate as the milk-flavored essence. Whey powder is added when a fermentation substrate is prepared, the whey powder is rich in whey protein, lactose, vitamins and mineral substances, and glycometabolism, protein metabolism and lipid metabolism pathways are activated in the fermentation process, so that a fermentation product not only can be rich in target milk flavor substances, but also can synergistically contain various flavor compounds such as esters, alcohols and aldehydes, and the richness of the flavor substances is improved; the anhydrous cream and the whey powder are prepared into the fermentation substrate, so that a comprehensive and balanced growth environment is provided for the German-type lactobacillus bulgaricus subspecies, and the fermentation efficiency is improved.

The present invention relates to a method for producing health-functional milk, comprising the step of selecting healthy dairy cows suitable for raw milk production by analyzing their health status after performing a comprehensive health examination including blood tests on dairy cows, and milking the selected dairy cows while feeding them a feed for constitutional improvement according to the lactation period after calving; wherein the comprehensive health examination includes protein metabolism, energy metabolism, liver function, and mineral metabolism. Accordingly, compared to milk produced by conventional methods, it can exhibit effects such as a fresh taste, reduced off-flavors and fishy tastes, and immune activity.

The present application relates to the field of food or medicine, and particularly relates to a kind of food-medicinal Chinese medicine composition and Chinese medicine preparation, preparation method and application.The raw materials of the food-medicinal Chinese medicine composition of the present application include yam, fuling, radix puerariae, medlar, mulberry leaf, hawthorn, cassia, polygonatum and gardenia.The food-medicinal Chinese medicine composition of the present application takes "clearing heat and drying dampness, benefiting qi and nourishing yin, promoting blood circulation to remove meridian obstruction" as the basic principle, adjusts the function of viscera, improves sugar, lipid and protein metabolism, promotes the recovery of the body environment to normal, and fits the basic pathogenesis of the middle and late stage of type 2 diabetes, and has accurate treatment effect.

The present invention relates to the field of feature extraction technology, specifically to a feature extraction method and system for CAR-NK cell data, comprising the following steps: collecting CAR-NK cell samples, performing protein analysis on the CAR-NK cell samples in batches, recording changes in protein expression in each batch of CAR-NK cell samples, and performing quantitative analysis on metabolite samples to obtain metabolite concentration data, and standardizing the concentration data to obtain basic data of the cell samples. In the present invention, by quantitatively analyzing proteins and metabolites in CAR-NK cell samples, the response characteristics of cells under different immune environments can be fully captured, immune response grouping and cluster analysis clarify the impact of the immune environment on cell function, the protein-metabolite interaction network reveals the interaction between molecules inside and outside the cell, and by screening and verifying immune markers, provides an accurate basis for immune function assessment. The quantitative analysis of immune reactivity characteristics can track the immune response process in real time, thereby improving the accuracy and depth of research.

The invention provides a Chinese herbal medicine feed additive for preventing and treating diarrhea of livestock and poultry and a preparation method thereof, and relates to the field of feed additives, the Chinese herbal medicine feed additive is composed of dandelion, mahonia bealei, rhizoma alismatis, gordon euryale seed, gallnut, Chinese yam, perilla leaf, pinellia ternate, red garden burnet, galangal, indigo naturalis, shiny cinquefoil root and bidens pilosa. The Chinese herbal medicine feed additive disclosed by the invention has broad-spectrum bacteriostatic activity on common pathogenic bacteria of livestock and poultry, can be used for remarkably reducing the occurrence rate of diarrhea of weaned piglets, reducing the levels of TNF-alpha and IL-6 in serum and increasing the content of IgA and IgG, and has an anti-inflammatory immune regulation effect; meanwhile, the feed additive can improve the content of TP and ALB in serum of weaned piglets, reduce the content of BUN, reduce the activity of ALT and AST, regulate protein metabolism disorder and protect liver and kidney functions; besides, the additive can optimize the intestinal microecological balance of weaned piglets, effectively prevent and treat diarrhea, and provide a safe and efficient prevention and control strategy for the diarrhea problem of livestock and poultry in large-scale breeding.

The invention discloses a method for analyzing an action mechanism of a breast nodule removing tablet. The method comprises the following steps: identifying chemical components of the breast nodule removing tablets; predicting effective target spots of the chemical components based on network pharmacology, and predicting target spots of hyperplasia of mammary glands; and performing clustering analysis and other operations on the relative content of differential proteins in the mammary gland hyperplasia model before and after the breast nodule removing tablets are taken based on proteomics. According to the application, a method for integrating multi-dimensional data through a'chemical component-target network-omics technology 'is used for discussing the action mechanism of the breast nodule removing tablets, important proteins, metabolites or pathways are screened out, research targets are more easily locked, and the action mechanism of the traditional Chinese medicine compound breast nodule removing tablets is disclosed.

The invention provides a feed for improving production performance of dairy cows and a preparation method of the feed. The feed comprises the following components in percentage by mass: 17.35% of alfalfa hay, 1.29-7.09% of beet grain meal, 19.84-23.44% of corn, 3.6-5.8% of whole cotton seeds, 0.82% of sodium bicarbonate, 29.57% of silage and the balance of 21.73% of concentrate supplement, and the silage comprises corn silage and tomato seedling silage. The preparation method of the feed is rapid and convenient, and the prepared feed can improve the milk fat correction milk yield, milk yield, feed conversion rate and milk protein rate of dairy cows, has positive influence on the protein metabolism capability, has no damage to the liver and kidney of an organism, and can improve anti-inflammatory factors and milk quality.

The invention relates to the technical field of feature extraction, in particular to a feature extraction method and system for CAR-NK cell data, and the method comprises the following steps: collecting CAR-NK cell samples, carrying out protein analysis on the CAR-NK cell samples in batches, recording expression change of protein in each batch of CAR-NK cell samples, carrying out quantitative analysis on metabolite samples, and obtaining metabolite concentration data. And carrying out standardization treatment on the concentration data to obtain basic data of the cell sample. According to the method, the protein and the metabolite in the CAR-NK cell sample are quantitatively analyzed, the reaction characteristics of the cell in different immune environments can be comprehensively captured, the influence of the immune environments on the cell function is clarified through immune response grouping and clustering analysis, and the protein-metabolite interaction network reveals the interaction of molecules inside and outside the cell, so that the immunologic function of the cell is enhanced. By screening and verifying immune markers, an accurate immune function evaluation basis and quantitative analysis of immune reactivity characteristics are provided, the immune reaction process can be tracked in real time, and the research precision and depth are improved.

The present invention provides a document analysis method capable of presenting MeSH and the like related to a specific physiological activity, work mechanism, functionality, and influence thereof on a living body from a target substance such as a protein, a metabolite, or a nucleic acid obtained from multi-omics analysis by visualizing an analysis result with a unique index.SOLUTION: The method includes at least one of a physiological activity / functionality prediction step of setting the number of selected substances used as an index for predicting a specific physiological activity or functionality and predicting the specific physiological activity or functionality, an enrichment analysis step of performing QPEA registered in a database and quantifying the degree of coincidence between a past research result and a selected substance having a significant difference in an actual research by ES, and a two stage enrichment analysis step of performing two stage enrichment analysis using at least one statistical index selected from ES, NES, and ER.SELECTED DRAWING: Figure 1

The application belongs to the technical field of microorganisms, discloses a Pseudomonas putida and application of the Pseudomonas putida as fish probiotics, and specifically discloses a Pseudomonas putida Pseudomonas putida CD, the strain has strong fat utilization capacity, can produce various beneficial extracellular metabolites, can promote fish lipid, carbohydrate and protein metabolism and weight gain, and can also improve the iron content and hemoglobin content in fish tissues. The Pseudomonas putida strain CD has good biological safety and has broad development prospects in the application as fish probiotics.

The present disclosure provides compositions, kits, and methods to protect organs by inducing acquired cytoresistance without causing injury to the organ. The compositions, kits, and methods utilize heme proteins, iron and / or vitamin B12 and, optionally, agents that impact heme protein metabolism.

The invention discloses a preparation method of freeze-dried zizania latifolia. The wild rice shoots are fermented by using phanerochaete chrysosporium, trametes hirsutum and inonotus subsp.nigrum, lignin in the wild rice shoots is effectively decomposed, and then the quality of the dried wild rice shoots is maintained through vacuum freeze drying. The freeze-dried zizania latifolia prepared by the method disclosed by the invention not only can prevent the zizania latifolia from being lignified, but also can promote saccharides, lipids and proteins to be metabolized into volatile flavor substances, so that the flavor of the zizania latifolia is improved.

The effects of the present invention include the effect of improving the ability to degrade soybean protein, and production activity of branched-chain amino acids such as valine, isoleucine, and leucine, as well as production activity of other amino acids including threonine, glycine, tyrosine, and lysine. Thus, effects of sarcopenia prevention and antioxidant activity can be obtained, and the effects of maintaining protein metabolism balance and ameliorating sarcopenia through muscle synthesis can also be obtained. In addition, compared to when each of the strains used in a strain mixture was used alone, the strain mixture exhibited improved autoaggregation, hydrophobicity, and adhesion to the intestines. Thus, when the strain mixture of the present invention is ingested, the corresponding strains can remain in the intestines for a long period of time.

The present disclosure can improve the degree of soy protein proteolysis and the ability to produce valine, isoleucine, and leucine, which are branched-chain amino acids, as well as other amino acids, such as threonine, glycine, tyrosine, and lysine. Accordingly, the present disclosure can prevent sarcopenia and has antioxidant activity. Also, the present disclosure can maintain protein metabolic balance and relieve sarcopenia by muscle synthesis.The self-aggregation, hydrophobicity, and intestinal adhesion of a mixed strain is improved compared to a case where a single strain used for the mixed strain is used. Therefore, when the mixed strain of the present disclosure is ingested, the strain can remain for a long time in the intestine.

The invention relates to the technical field of traditional Chinese medicine, in particular to a traditional Chinese medicine composition for improving muscle attenuation and preparation and application thereof. The traditional Chinese medicine composition for improving muscle attenuation comprises a rhodiola rosea extract, an American ginseng extract and a lucid ganoderma extract. The traditional Chinese medicine composition is beneficial to adjusting balance of muscle protein synthesis and decomposition, further protects muscle protein metabolism from two aspects of resisting inflammation and relieving insulin resistance, reduces damage to muscles by resisting oxidation and protecting mitochondrial functions, and can achieve the effects of maintaining and improving muscle quality and preventing and relieving muscle attenuation.

The effects of the present invention include the ability to decompose soybean protein, the ability to produce valine, isoleucine, and leucine, which are branched chain amino acids, and the ability to produce threonine, glycine, tyrosine, and lysine, which are other amino acids, are improved. As a result, it is possible to achieve the effects of preventing sarcopenia and antioxidant activity, and also achieve the effect of improving sarcopenia by maintaining a protein metabolic balance and muscle synthesis. Furthermore, the self-aggregation property, hydrophobicity, and intestinal adhesion of the mixed strain are improved compared to the case in which each strain used in the mixed strain is used alone, and therefore, when ingestion of the mixed strain of the present application, it is possible to obtain the effect of long-term retention of the corresponding strain in the intestinal tract.

The invention belongs to the technical field of traditional Chinese medicine pharmacology, and discloses a method for analyzing an action target and mechanism of artemisinin for improving ischemic cerebral apoplexy based on network pharmacology and a molecular docking technology, and the method comprises the following steps: S1, observing a protection effect in an ischemic cerebral apoplexy in-vitro H2O2 induced oxidative stress model, and obtaining a drug effect; s2, acquiring a drug-disease common target spot; s3, constructing a protein internetwork; s4, acquiring a core target spot of artemisinin acting on the cerebral arterial thrombosis; s5, exploring a potential mechanism of artemisinin acting on cerebral arterial thrombosis through KEGG pathway enrichment analysis and GO function enrichment analysis; and S6, exploring an action mechanism of artemisinin for improving cerebral arterial thrombosis based on a molecular docking technology. According to the invention, an in-vitro experimental model and multiple omics data of genes, proteins, metabolites and the like are integrated, the effect of drugs in diseases is observed, and a disease-related biomolecular network is constructed.

The invention provides a Chinese herbal medicine feed additive for preventing and treating diarrhea of livestock and poultry and a preparation method thereof, and relates to the field of feed additives, the Chinese herbal medicine feed additive is composed of dandelion, mahonia bealei, rhizoma alismatis, gordon euryale seed, gallnut, Chinese yam, perilla leaf, pinellia ternate, red garden burnet, galangal, indigo naturalis, shiny cinquefoil root and bidens pilosa. The Chinese herbal medicine feed additive disclosed by the invention has broad-spectrum bacteriostatic activity on common pathogenic bacteria of livestock and poultry, can be used for remarkably reducing the occurrence rate of diarrhea of weaned piglets, reducing the levels of TNF-alpha and IL-6 in serum and increasing the content of IgA and IgG, and has an anti-inflammatory immune regulation effect; meanwhile, the feed additive can improve the content of TP and ALB in serum of weaned piglets, reduce the content of BUN, reduce the activity of ALT and AST, regulate protein metabolism disorder and protect liver and kidney functions; besides, the additive can optimize the intestinal microecological balance of weaned piglets, effectively prevent and treat diarrhea, and provide a safe and efficient prevention and control strategy for the diarrhea problem of livestock and poultry in large-scale breeding.

The invention discloses a yak maintenance and growth net protein demand amount calculation method, and relates to the technical field of livestock breeding, and the method comprises the following steps: obtaining basic data of adult yaks through experiments, calculating the maintenance net protein demand amount, and building a growth net protein demand amount model; according to the method, the gradient feeding test, the nitrogen metabolism test and the comparative slaughtering test are integrated, protein metabolism and body composition first-hand data of the plateau adult yaks are directly obtained, an accurate mathematical model is established according to the data, the calculation result directly reflects the real physiological needs of the yaks, scientificity is remarkably enhanced, and based on the model, the real physiological needs of the yaks are directly reflected. By means of the method, the net protein requirement amount for maintaining the net protein and the net protein requirement amount for growth required by target weight gain of the yaks of any weight can be accurately calculated, the feed utilization efficiency can be greatly improved, the feeding cost can be reduced, nutrient emission can be reduced, and the growth performance of the yaks can be optimized.

Various aspects relate to a cell-free protein expression method. The method includes exposing a microorganism to substantially anaerobic growth conditions to produce a conditioned microorganism. The method further includes lysing the conditioned microorganism to produce a lysate. The method further includes combining the lysate with a nucleic acid and producing a protein of interest a metabolic pathway, a molecule, or a mixture thereof from the lysate.

The invention relates to the technical field of proteometabolism, and provides a proteometabolism method based on combination of complementary enzyme digestion, which comprises the following steps: firstly, selecting a key rate-limiting enzyme in a target metabolic pathway, modifying a coding sequence of the key rate-limiting enzyme, and inserting a specific protease recognition sequence; then, a pair of functional complementary protease fragments is constructed, expression strategies of the protease fragments in cells are designed respectively, one fragment achieves constant low-level expression, and the other fragment is expressed under the induction of a specific signal; when the two fragments coexist in cells, the two fragments can be combined to form an enzyme complex with cleavage activity, so that a target sequence on a rate-limiting enzyme is recognized and cleaved. And after the signal is removed, the enzyme complex is gradually decomposed, and the expression level of the target rate-limiting enzyme is recovered. In addition, according to the method, metabolic modeling and protein expression cost parameters are combined, and the expression proportion of protease to rate-limiting enzyme is synergistically optimized, so that the system stability and regulation efficiency are improved.