30 results about "Serum product" patented technology

The invention discloses a serum-free medium and a preparation method thereof, and belongs to the technical field of biochemistry. The preparation method comprises the following steps: 1, preparing an amino acid stock solution, a glutamine stock solution, a tryptophan and tyrosine disodium salt stock solution, a vitamin stock solution, an inorganic salt stock solution, a trace element stock solution and an insulin stock solution; and 2, adding a proper amount of potassium chloride, sodium chloride, and the amino acid stock solution, the tryptophan and tyrosine disodium salt stock solution, the vitamin stock solution, the inorganic salt stock solution, the trace element stock solution and the insulin stock solution which are prepared in step 1 into ultrafine water, adding sodium bicarbonate, carrying out mixing and dissolving to obtain a mixed solution, and adding the glutamine stock solution which is unfrozen by 37 DEG C water bath into the mixed solution before use in order to prepare the serum-free medium. The serum-free medium makes cell proliferation normal and the activity stable, is non-obviously different from a medium containing 10% of a finished calf serum product, has an obviously lower protein content than routine media, simplifies the extraction technology, and reduces the production cost.