30 results about "Serum product" patented technology

The present invention relates to a plasma product or a serum product with an extremely low risk of viral contamination and a method for producing the same. Before treating plasma or serum to be used as a raw material for producing a plasma product or a serum product using a virus removal membrane, leucocytes contaminating the blood are removed. Thus, a plasma product or a serum product with an extremely low risk of viral contamination can be efficiently produced while preventing clogging. Since clogging scarcely arises, it is possible to carry out efficient filtration without applying an elevated pressure as the filtration proceeds.

Analysis of a goat serum product with many therapeutic effects is described. The product is identified as containing proopiomelanocortin (POMC) and Corticotropin releasing factor (CRF) peptides, as well as breakdown products of these peptides. We describe methods of treatment of diseases including cancers, multiple sclerosis, and neural disorders using these peptides and their products, as well as medicaments including such peptides and methods of producing the peptides.

The invention belongs to the field of antibodies, and in particular relates to an anti-human abnormal prothrombin antibody and the use of the antibody in preparing a hepatocellular carcinoma detection kit. The anti-human abnormal prothrombin antibody of the present invention can specifically bind human abnormal prothrombin, can further avoid interference from prothrombin in serum or plasma from the raw material level, and can further avoid abnormal blood coagulation from other species in animal serum products The interference of zymogens avoids related risks from the source, reduces the difficulty of reagent development, and has a good application prospect.

The invention discloses a method for preparing a large-capacity serum antibody, which comprises the following steps: first collecting immunized fresh blood, collecting the plasma, and freezing and storing it for later use; then filtering the collected plasma, and collecting the filtrate to obtain a primary filtered serum product; Add thrombin to the prepared primary filtered serum product, stir evenly, and then apply it warmly for 0.5 to 4 hours at 20 to 37°C, then filter with filter paper with a pore size of 0.22 to 0.88 μm, collect the filtrate, and obtain serum antibodies; using the present invention The serum antibody prepared by the method has large quantity, good quality and good stability, is suitable for the preparation of large-capacity serum antibody, and has a good market prospect.

The invention discloses a fluorescent quantitative PCR kit for detecting bovine adenovirus type 3 and its application. The kit contains: a) a DNA extraction reagent, b) a hot-start Taq DNA polymerase, c) a primer and a TaqMan probe, d) ) Standard positive DNA template, e) PCR fluorescent quantitative reaction solution, characterized in that: the primer sequences are respectively sense primer: 5'-CCTGAATTCTCTTGCAGCCAGA-3', antisense primer: 5'-CCTACCGAACCGACGCAGAT-3', and the amplicon size is 100bp, the fluorescent probe sequence is: 5′-FAM-TGAGAAGGTACTCCTCGTCGCTGGACCA-TAMRA-3′, the 5′ end of the probe is labeled with the fluorescent emitting group FAM, and the 3′ end is labeled with the fluorescent quenching group TAMRA, and the standard positive DNA template is prepared by The pGEM-T vector inserted with the 100bp fragment of the adenovirus type 3 pol protein was transformed into Escherichia coli DH5α, and after multiplication, the plasmid was extracted and prepared, and quantified by measuring A260 with a UV spectrophotometer and 10-fold serial dilution. Efficient and convenient real-time monitoring of bovine adenovirus type 3 contamination in serum products can be widely used in epidemiological investigations of the virus infection, and can also provide technical support for related basic research, with broad application prospects.

The present invention relates to the use of a pharmacologically active blood serum product producible by a method comprising electrostimulation of a non-human animal, withdrawal of blood from said animal, isolation of serum from said blood, and gamma irradiation of said serum in the treatment of stroke, preferably ischemic stroke.

The present invention relates to a method for preparing a blood serum product, the blood serum product and a pharmaceutical composition comprising said blood serum product as well as uses thereof in the treatment of various diseases and conditions, including epileptic seizures and apoplexy.

The invention discloses a bovine parvovirus detecting fluorescence quantitative PCR kit and an application thereof. The kit comprises: a) a DNA extraction reagent, b) a hot start Taq DNA polymerase, c) a primer and a TaqMan probe, d) a standard positive DNA template, and e) a PCR fluorescence quantitative reaction solution. The kit is characterized in that: primer sequence is a sense primer: 5'-CCAGTACCAGGAAACGGAGAC-3', and an antisense primer: 5'-GCATGTATTCCGGTCTCCAA -3', and the size of an amplicon is 118 bp; the sequence of a fluorescence probe is: 5'-FAM-CCTCAACATCTACGTCACCGGACAA-TAMRA-3',the 5' end of the probe is marked with a fluorescence emission group FAM, the 3' end is marked with a fluorescence quenching group TAMRA, the standard positive DNA template transforms a colon bacillus DH5 alpha by a pGEM-T carrier inserted with bovine parvovirus VP3 protein coding zone 118 bp segment, plasmid is extracted after proliferation, an A260 ration is measured in an ultraviolet spectrophotometer, and the plasmid is diluted by 10 times of gradient. The fluorescence quantitative PCR kit, applied to the epidemiology investigation of cow bovine parvovirus infection, can efficiently and conveniently monitor the bovine parvovirus pollution in serum products in real time, and is widely applicable to the epidemiology investigation of bovine parvovirus infection.