The invention discloses a constant-temperature type hot-film shear stress micro-sensor closed-loop feedback control system, and belongs to the field of sensor measuring instruments. The adopted technical scheme of the invention comprises a hardware circuit design and an algorithm design of the hot-film shear stress micro-sensor closed-loop feedback control system. The hardware circuit is formed bya fixed resistor1, a flexible hot-film shear stress micro-sensor 4, a simulation operational amplifier 6, an instrument operational amplifier 7, a simulation multiplier 8, a microcontroller 9, and alow-noise amplifier 10. The algorithm design comprises threshold setting, design of an integration link and design of a proportion link. According to the constant-temperature type hot-film shear stress micro-sensor closed-loop feedback control system, through the design form of four-wire system measurement of the sensor resistance, the measuring error caused by a lead wire resistance of the hot-film shear stress micro-sensor is greatly reduced, and the calibration difficulty of the hot-film shear stress micro-sensor in a practical application process is reduced; besides, through dynamic and real-time adjustment of the algorithm of the microcontroller 9, the problems that according to a conventional design scheme, a self-excitation state is entered due to large-range change of the flow velocity of a flow field, and normal operation is failed are avoided.