The invention provides an anti-mosquito disinfectant and a preparation method thereof, mainly comprising chlorhexidine acetate, vitamin B5, comfrey extract, lavender oil, citronella essential oil, tea tree oil, vitamin E, absolute ethanol, glycerin, Tween, Composed of water and methylene blue, the invention has no skin irritation, is suitable for local antibacterial disinfection, and can effectively kill various bacteria and harmful microorganisms on the skin. At the same time, it can effectively prevent mosquito bites, relieve discomfort after mosquito bites, and eliminate itching after mosquito bites.