The invention discloses a TWDM-PON (Time Wavelength Division Multiplexing-Passive Optical Network) ONU (Optical Network Unit) implementation device and method based on a COTF (Cyclical Optical Tunable Filter), and relates to the field of PON access. The ONU implementation device comprises an optical coupler, a circulator, a gain medium, a BPF (Band Pass Filter) 1, an isolator, a WDM (Wavelength Division Multiplexing), a COTF, a COTF control circuit, a BPF 2, a receiver, a signal modulating circuit and a burst control circuit; the COTF is connected with the COTF control circuit; the optical coupler, the COTF, the WDM, the BPF2 and the receiver are connected in sequence to form a downlink chain jointly; the optical coupler, the circulator, the BPF1, the isolator, the WDM, and the COTF form a closed loop; the circulator is connected with the gain medium; the gain medium is further connected with the signal modulating circuit and the burst control circuit respectively to form an uplink chain jointly. By adopting the the device and the method provided by the invention, the selectable wavelength of the ONU receiving end and the changeable wavelength of the sending end can be realized; the requirement of the TWDM-PON system to the ONU is satisfied; the existing investment of an operator is protected, and the construction cost of the network is saved.