The invention discloses an air conditioning system used for a crop culture room. The air conditioning system comprises a culture room which is internally provided with an air supply box and an air return box, and an independent air conditioning unit which is arranged outside the culture room, wherein the wall body inside the culture room is internally nested with a plurality of wall coils from bottom to top or from top to bottom, one side of the independent air conditioning unit is correspondingly communicated with the air supply box, the air return box and the wall coils arranged in the culture room through a primary air pipe, an exhaust pipe, a water supply pipe and a water return pipe, and the other side of the independent air conditioning unit is communicated with the atmosphere environment through an environment air inlet, an environment air outlet, a primary air inlet and an exhaust port, and is provided with a humidification water pipe. When the air conditioning system is used for heating, a heat pump operation mode is started, and when the air conditioning system is used for refrigerating, a refrigerating operation mode is started. Due to the adoption of the air conditioning system, the culture room is more suitable for the natural environment characteristic for crop growth, and is convenient to arrange and operate, safe and sanitary in production and low in energy consumption.