The invention discloses a blumea oil transdermal patch, a preparation method thereof and a method for detecting active components contained in the blumea oil transdermal patch. The blumea oil transdermal patch comprises a back lining material, a medicated layer and a protective film, wherein the medicated layer comprises a humectant, a filling agent, a patch skeleton, a cross-linking regulating agent, a cross-linking agent, a thickening agent, an emulsifying agent and blumea oil. The blumea oil transdermal patch disclosed by the invention has the advantages of high medicine permeability and good adhesion without stimulation. The preparation method of the blumea oil transdermal patch comprises the following steps: respectively preparing a humectant phase composed of the cross-linking agent, the cross-linking regulating agent, the humectant and the patch skeleton and a water phase composed of the blumea oil, the emulsifying agent, ultrapure water, the filling agent and the thickening agent; slowly adding the humectant to the water phase, uniformly stirring and mixing to obtain a colloid, coating the colloid to the back lining material, adhering the protective film, and standing to solidify for 6-12 hours. According to the method for measuring the pharmacological active components contained in the blumea oil transdermal patch, an HP-INNOWAX capillary chromatographic column is adopted, the flowing phase is N2, the flow velocity is 0.7-1 mL.min<-1>, and the content of eucalyptol, camphor and borneol can be accurately measured.