The invention discloses a control system and a control method for an agaricus bisporus picking device, belongs to the technical field of agricultural engineering, and solves the problem that the existing mushroom picking technology is low in recognition degree, accuracy and picking rate. The system comprises an electric control loop and a pneumatic loop, the electric control loop comprises a main controller, a picking module, an undercutting module, a moving module and a visual module, and the picking module, the undercutting module, the moving module and the visual module are respectively connected with the main controller; the picking module comprises a picking motor, a motor driver A, a two-position three-way reversing valve, a relay A and a tension and compression sensor; the moving module is used for moving the picking module; the pneumatic loop comprises an air pump, a filtering-pressure regulating-lubricating unit, a four-position flow control valve seat, a Z-direction double-acting pneumatic cylinder, an undercut double-acting pneumatic cylinder, a negative pressure generator and a suction cup. The mushroom picking device is suitable for picking clustered and high-density mushrooms.