The invention belongs to the field of virus detection, and particularly relates to a real-time fluorescence RT-PCR method for quantitatively detecting a human-infected avian influenza virus (A/H7N9). According to comparative analysis with an existing human-infected H7N9 virus in a Genbank database, it is shown that a synthesized and designed primer and probe are completely conservative to the epidemic H7N9 virus. According to the method, a higher detection sensitivity is achieved on the condition that five genes are copied in each reaction, a cross reaction on 17 common respiratory tract infection viruses does not occur, and the H7N9 can be specifically detected; contrast detection between the method and a national CDC detection method is conducted on 80 clinical specimens, double-check is conducted on 13 inconsistent specimens by adopting rRT-PCR recommended by WTO for detecting an influenza A virus, detection results are all positive, and it is shown that the detection results are accurate and reliable. The method can conduct quantitative analysis on the copy number of the H7N9 virus in the clinical specimens, the specificity, the sensitivity and the accuracy are achieved, the method is suitable for large-scale detection of the clinical specimens, and an important basis is provided for optimizing clinical treatment strategies.