The invention belongs to the technical field of microfluidic
spinning, relating to an integrated microfluidic
spinning chip and a method for preparing regenerated silk
fibroin by using the same. The integrated microfluidic
spinning chip is of a three-layer structure, wherein the upper layer and the lower layer are provided with
polydimethylsiloxane membranes of grooves, and the grooves are adhered with a middle
dialysis membrane to form an upper
microfluidic channel and a lower
microfluidic channel. The depths of the two microfluidic channels are the same, and the widths gradually become small. The method for preparing the regenerated silk
fibroin disclosed by the invention comprises the following steps: injecting spinning liquid in the upper channel and the lower channel at
room temperature; injecting the mixed liquid of water-absorbent resin and
ion adjusting liquid in the lower channel; and after the spinning liquid is adjusted via ions,
cut, stretched and concentrated, extruding from an outlet, then solidifying in air as threads, and then winding the threads on rolls. The
microfluidic chip disclosed by the invention can be used for simulating complex flow fields during the biological spinning process, and realizing the operations of the spanning liquid, such as
cutting, stretching, component adjusting and concentrating. Compared with the spinning technology using a dry method, the method disclosed by the invention can be used for preparing good regenerated silk
fibroin.