The invention discloses a single-piece fireproof glass detection device. The device comprises a base, a supporting block, a threaded rod, a first screw rod, a mounting plate, a second screw rod and adischarging mechanism, a sliding groove is formed in the top end of the base, a rubber pad is embedded and fixed to the top end of the supporting block, a connecting rod is fixed to the bottom of thesupporting block, and the threaded rod penetrates through a mounting hole; the bottom of the first lead screw penetrates through the top end of the mounting frame, a winding motor is fixed to the right side above the mounting plate, the right side of the second lead screw penetrates through the top end of the left side of the transverse plate, a concave block is fixed to the top end of the right side of the back face of the sliding rail, a telescopic motor is fixed to the left side of the concave block, the output end of the telescopic motor is fixedly connected with the right end of a secondsliding block, the telescopic motor is electrically connected with a controller, and a connecting block is welded and fixed to the bottom of a wire rope. The single fireproof glass detection device isprovided with the threaded rod, and the fireproof glass is fixed through the threaded rod, so the situation that the fireproof glass is stressed to bounce upwards, and consequently impact resistancedata of the fireproof glass is inaccurate is prevented.