The object of the present invention is to provide a method for the detection and / or quantification of the 14-3-3 proteins or their isoforms for early stage diagnosis of TSE-diseases, which method allows to perform the diagnosis in the living
organism. It is furthermore an object, to detect a
contamination of the sample by the parallel determination of a second
antigen. This object according to the invention is solved by making use of the biochemical characteristics of the members of the 14-3-3
protein family, which bind to specific
amino acid motifs like X(n)-XSXXSXXSX-X(n) or to the motif RSXpSXP (SEQ ID NO: 12) within peptides or proteins. For determining one or more isoforms or the entirety of the 14-3-3
protein(s) and for specific binding, one uses modified
solid phases like e.g. microtiter plates, which are coated with a synthetic or natural
peptide containing a binding motif for 14-3-3 proteins, e.g. a chemically synthesised
peptide having the motif CAALPKINRSApSEPSLHR (SEQ ID NO: 1). After the addition of the extracts or bodily fluids to be investigated the detection and quantification of the generated
peptide-14-3-3
protein complexes is accomplished by means of labeled antibodies. The use of the 14-3-3
protein family and / or of individual isoforms of the 14-3-3 proteins according to the invention can be employed as an effect monitor or biomonitor in aquatic invertebrates after environmental effects like the presence of polychlorinated biphenyls (PCBs), (xeno)estrogens etc. Moreover, the method can be used for early diagnosis of TSE-diseases like e.g. Creutzfeldt-Jakob
disease (CJD) and its novel form (variant) in young persons (vCJD) and
Bovine Spongiform Encephalopathy (BSE) or comparable diseases. Thus a
diagnostic marker (surrogate marker) is available, which can be used in the living
organism as a screening marker, confirmation marker or single marker.