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Use of 14-3-3 proteins and a method for determining the same in the fluids or tissues of organisms

The object of the present invention is to provide a method for the detection and/or quantification of the 14-3-3 proteins or their isoforms for early stage diagnosis of TSE-diseases, which method allows to perform the diagnosis in the living organism. It is furthermore an object, to detect a contamination of the sample by the parallel determination of a second antigen. This object according to the invention is solved by making use of the biochemical characteristics of the members of the 14-3-3 protein family, which bind to specific amino acid motifs like X(n)-XSXXSXXSX-X(n) or to the motif RSXpSXP (SEQ ID NO: 12) within peptides or proteins. For determining one or more isoforms or the entirety of the 14-3-3 protein(s) and for specific binding, one uses modified solid phases like e.g. microtiter plates, which are coated with a synthetic or natural peptide containing a binding motif for 14-3-3 proteins, e.g. a chemically synthesised peptide having the motif CAALPKINRSApSEPSLHR (SEQ ID NO: 1). After the addition of the extracts or bodily fluids to be investigated the detection and quantification of the generated peptide-14-3-3 protein complexes is accomplished by means of labeled antibodies. The use of the 14-3-3 protein family and/or of individual isoforms of the 14-3-3 proteins according to the invention can be employed as an effect monitor or biomonitor in aquatic invertebrates after environmental effects like the presence of polychlorinated biphenyls (PCBs), (xeno)estrogens etc. Moreover, the method can be used for early diagnosis of TSE-diseases like e.g. Creutzfeldt-Jakob disease (CJD) and its novel form (variant) in young persons (vCJD) and Bovine Spongiform Encephalopathy (BSE) or comparable diseases. Thus a diagnostic marker (surrogate marker) is available, which can be used in the living organism as a screening marker, confirmation marker or single marker.
Owner:MUELLER WERNER +1

Data Gathering Device and Method of Removing Contaminations from a Borehole Wall of a Well Before In Situ Gathering of Formation Data from the Borehole Wall

Data gathering device for in situ gathering of formation data from a borehole wall of a well in an open borehole comprises at least one movable measuring probe structured to be movable out from a well pipe to be able to establish contact with the borehole wall; at least one activation device structured in a manner allowing it to cause said measuring probe movement; at least one measuring sensor which, for measuring at least one formation-associated parameter, is connected to the measuring probe; and at least one data registration device which, for registration of measured parameters, is connected to the measuring sensor. The distinctive characteristic of the data gathering device is that it also comprises at least one suction chamber hydraulically connected to the measuring probe; wherein the suction chamber is structured in a manner allowing it to carry out non-motorized suction of contaminations from the borehole wall before the gathering of formation data is initiated; and wherein the suction chamber is connected to a release means for controlled activation of said suction. This suction removes said contaminations and thus brings about the best possible contact with the borehole wall during gathering of formation data.
Owner:SHORE TEC CONSULT
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