The invention discloses an anti-interference pulse signal conditioner. The anti-interference pulse signal conditioner comprises a first capacitor, a second capacitor, a third capacitor, a fourth capacitor, a first resistor, a second resistor, a third resistor, a fourth resistor, a fifth resistor, a diode, a first power source, a second power source and an amplifier. The anti-interference pulse signal conditioner can filter and amplify input signals, remove direct current and extremely-low frequency interference signals, restrain high frequency interference, amplify useful pulse signals, and eliminate bias voltage, is simple in circuit structure and is suitable for being used for pulse testing instruments.