The invention relates to the field of breeding environment control, and particularly discloses a precise local ventilation airflow organizing mechanism for a livestock breeding house and an application of the precise local ventilation airflow organizing mechanism. The precise local ventilation airflow organizing mechanism comprises an air supply assembly, an air return assembly and an exhaust assembly. The air supply assembly comprises an air supply main pipe, an air supply branch pipe and an air supply opening, and the air supply opening is formed in the upper portion of a breeding fence and faces downwards; the air return assembly comprises an air return main pipe, an air return branch pipe and an air return opening. The air return opening is formed in the middle or the upper portion of the breeding fence. The exhaust assembly comprises an exhaust channel and an exhaust opening, the exhaust channel is arranged on one side or two sides of a manure pit, the exhaust opening is formed in the upper portion of the side wall of the manure pit, and the exhaust channel communicates with the manure pit through the exhaust opening. According to the precise local ventilation airflow organizing mechanism for the livestock breeding house and the application of the precise local ventilation airflow organizing mechanism, through precise local short-range air supply, air return and air exhaust and scientific and reasonable air distribution, ventilation distribution is more uniform, waste gas backflow is reduced, the concentration of harmful gas in a livestock living area is effectively reduced, the livestock isolation effect is optimized, cross contamination and germ transmission are reduced, and the ventilation quantity and energy consumption are effectively reduced.