34results about How to "Wide range of temperature tolerance" patented technology

The invention relates to a breeding method for a new marine backcrossed scallop variety. The breeding method is characterized in that: a best individual is chosen from the first filial generation family or group of Argopecten irradians and Argopecten purpuratus, and is first backcrossed with Argopecten irradians to establish a backcrossed family or group, a best hermaphrodite individual is then chosen from the backcrossed family or the backcrossed group with excellent performance, at least three generations of selved families are then bred in succession, and thereby the new marine backcrossed scallop variety which has the characteristics of stable heredity, fast growth, big individual and wide temperature tolerance range is obtained. The new marine backcrossed scallop variety bred by the breeding method has the characteristics of both Argopecten irradians and Argopecten purpuratus, i.e. fast growth, big individual, wide temperature tolerance range and stable heredity, and can be cultured in northern sea areas such as the sea areas of Tsingtao and Dalian, and moreover, the current yield of the new marine backcrossed scallop variety is increased by more than 30 percent in comparison with the current yield of Argopecten irradians.

The invention relates to a culture method of a new hybrid variety of Argopecten irradias and Argopecten purpuratus. The culture method comprises the following steps of fertilizing Argopecten irradias eggs with Argopecten purpuratus sperms to build multiple hybrid first filial generation lines, selecting excellent hybrid lines according to phenotype of each hybrid line, selecting excellent hermaphroditic individuals from the excellent hybrid lines, maturing the excellent hermaphroditic individuals, and then carrying out self-fertilization to build multiple selfing lines; selecting excellent lines from the selfing lines, selecting excellent hermaphroditic individuals from the excellent lines, carrying out selfing and selecting; and carrying out selfing for more than three generations according to the same method to obtain the new hybrid variety of Argopecten irradias and Argopecten purpuratus. The obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has stable inheritance, rapid growth, large size and wide temperature tolerance range. The culture method also can be implemented by adopting a colony culture method. Since the obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has the excellent characteristics of Argopecten irradias and Argopecten purpuratus at the same time, the new hybrid variety can be cultivated in Qingdao, Dalian and other northern sea areas and the yield is improved by more than 30% as compared with the yield of bay scallop.

The invention discloses a multiplex amplification detection kit for detecting 60 loca of autosomes and Y-chromosomes simultaneously. The 60 loca are amplified in a multiplex manner through a polymerase chain reaction, and amplification products of the loca are detected by using a gene sequencer. The kit can be used for detecting the 60 loca of the autosomes and the Y-chromosomes simultaneously, which is a case that most STR loca can be detected by a primary reaction with a capillary electrophoresis method at present, and databases can be built for autosome STR and Y-chromosome STR simultaneously by the primary reaction. The kit has strong adaption to biomaterials, namely, one kit can be used for amplifying various biomaterial samples, wherein different biomaterial samples comprise a male genome DNA extracted by a Chelex100 method, a magnetic bead extracting method or an organic extracting method and male blood or oral cells of human, which is / are collected by any one carrier of filterpaper, an FTA card, a cotton bud, gauze and the like. The kit has higher amplification specificity and higher thermal tolerance.

The invention relates to a composite amplification kit of 47 human autosome and Y chromosome loci and an application thereof. The invention provides a composite amplification system of the 47 human autosome and Y chromosome loci, which comprises specific primers for amplifying the 47 loci, wherein the 47 loci comprise 19 autosome STR loci, 27 Y chromosome STR loci and 1 sex recognition locus. The47 pairs of specific primers are subjected to grouping fluorescence labeling by utilizing a six-color fluorescence labeling technology, and the simultaneous high-efficiency, specific and sensitive amplification of the 47 human autosome and Y chromosome loci is achieved through the design and optimization of primer sequences and working concentrations. The detection result of the composite amplification system has high individual identification capability and good data compatibility, and can be used for paternity identification and individual identification in practice, so that the detection cost of human DNA typing is effectively reduced, and the detection working efficiency is improved.

The invention relates to a composite amplification kit for 44 human Y chromosome loci and application thereof. The invention provides a composite amplification system of the 44 human Y chromosome loci. The system includes specific primers for amplifying the 44 loci including 41 Y chromosome STR loci and 3 Y chromosome Indel loci. The 44 pairs of specific primers are subjected to group fluorescencelabeling by the six-color fluorescent labeling technology. Through design and optimization of the primer sequence and the working concentration, simultaneous efficient specific sensitive amplification of the 44 human Y chromosome loci is achieved. A detection result of the composite amplification system has high individual recognition capability and good data compatibility, and can be used in practice for paternity testing and individual recognition, thereby effectively reducing the detection cost of human DNA typing and improving the detection working efficiency.

The invention relates to a composite amplification kit for 25 human chromosome loci and application thereof. The invention provides a composite amplification system of the 25 human chromosome loci. The system includes specific primers for amplifying the 25 loci including 23 autosomal STR loci, 1 gender recognition locus and a Y chromosome Indel locus. The 25 pairs of specific primers are subjectedto group fluorescence labeling by the six-color fluorescent labeling technology. Through design and optimization of the primer sequence and the working primer concentration, simultaneous efficient specific sensitive amplification of the 25 human chromosome loci is achieved. A detection result of the composite amplification system has high individual recognition capability and good data compatibility, and can be used in practice for paternity testing and individual recognition, thereby effectively reducing the detection cost of human DNA typing and improving the detection working efficiency.

The invention discloses a high-strength antifreeze conductive polypyrrole hydrogel and a preparation method thereof, and belongs to the field of polymer materials. The high-strength antifreeze conductive polypyrrole hydrogel is obtained by in-situ chemical oxidative polymerization of a pyrrole monomer in a hydrogen bond-enhanced polymer network, multiple freezing and thawing cycles, and partial displacement of moisture in the gel by a low freezing point medium. The method is wide in raw material source and easy to operate and facilitates industrial production. The high-strength antifreeze conductive polypyrrole hydrogel prepared with the method has good electrical conductivity and mechanical properties, can withstand a relatively wide temperature range, and is unlikely to lose water, shrink and freeze. The high-strength antifreeze conductive polypyrrole hydrogel can withstand subzero 20 DEG C for 72 hours without being frozen, can be placed at 80 DEG C for 4 hours without drying and shrinking, and has an electrical conductivity of 0.5-1.5 S / m, a tensile strength of 0.5 MPa and a compressive elastic modulus of 200 kPa.

The invention discloses a multifunctional organogel, and a preparation method thereof. The raw materials of the multifunctional organogel comprise a solvent, a monomer, a crosslinking agent, an electrolyte salt, and an initiator; the volume ratio of the solvent to the monomer is controlled to be 1:5-5:1; the molar ratio of the crosslinking agent to the monomer is controlled to be 0.05 to 10%; themolar ratio of the initiator to the monomer is controlled to be 0.1 to 10%; and the concentration of the electrolyte salt in a mixed solution of all the raw materials ranges from 0.1 to 5mol / L. The multifunctional organogel possesses high transparency, high stretching performance, and high conductivity; the temperature resistance and the stability are better than those of conventional hydrogel materials; the temperature resistant range is very wide; the elongation at break is 1219% or higher; and the application prospect in the field of flexible electron and flexible machine is promising.

The invention relates to a culture method of a new hybrid variety of Argopecten irradias and Argopecten purpuratus. The culture method comprises the following steps of fertilizing Argopecten irradias eggs with Argopecten purpuratus sperms to build multiple hybrid first filial generation lines, selecting excellent hybrid lines according to phenotype of each hybrid line, selecting excellent hermaphroditic individuals from the excellent hybrid lines, maturing the excellent hermaphroditic individuals, and then carrying out self-fertilization to build multiple selfing lines; selecting excellent lines from the selfing lines, selecting excellent hermaphroditic individuals from the excellent lines, carrying out selfing and selecting; and carrying out selfing for more than three generations according to the same method to obtain the new hybrid variety of Argopecten irradias and Argopecten purpuratus. The obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has stable inheritance, rapid growth, large size and wide temperature tolerance range. The culture method also can be implemented by adopting a colony culture method. Since the obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has the excellent characteristics of Argopecten irradias and Argopecten purpuratus at the same time, the new hybrid variety can be cultivated in Qingdao, Dalian and other northern sea areas and the yield is improved by more than 30% as compared with the yield of bay scallop.

The invention discloses a Y-STR compound amplification detection kit marked by a novel fluorescent labeling method and a use method thereof. The Y-STR compound amplification detection kit performs compound amplification of 27 Y-STR loca with a compound amplification primer by adopting a polymerase chain reaction; the amplification product is detected by means of a gene sequencing instrument of a single channel or multichannel capillary tube; 27 Y-STR loca comprise 20 conventional low mutation rate loca and 7 fast mutant loca. The novel fluorescent labeling method comprises the steps of: marking a first alkaline group at the 5' end of the primer by a conventional dye; and marking the sixth alkaline group with a short excitation wavelength fluorescent dye to realize fluorescent energy transfer. The signal strength generated by the method is 3-11 times higher than that of the conventional method, and an amplification result which is more stable and balanced and higher in sensitivity can be obtained.

The invention discloses the application of a multifunctional organic gel as a conductive medium. The preparation raw materials of the multifunctional organic gel comprise a solvent, a monomer, a crosslinking agent, an electrolyte salt and an initiator, the volume ratio of the solvent to the monomer is 1:5 to 5:1, the molar ratio of the crosslinking agent to the monomer is 0.05% to 10%, the molar ratio of the initiator to the monomer is 0.1% to 10%, and the concentration of the electrolyte salt in a mixed solution of all the raw materials is 0.1 mol / L to 5 mol / L. When the organic gel is used asa conductive medium, since the organic gel has good electrical conductivity, a bulb can be lighted when the organic gel is used as a conductive line to energize the bulb, and an LED lamp bead can belighted when the organic gel is used as a conductive substrate to energize the LED lamp bead attached to the conductive substrate.

The invention relates to a breeding method for a new marine backcrossed scallop variety. The breeding method is characterized in that: a best individual is chosen from the first filial generation family or group of Argopecten irradians and Argopecten purpuratus, and is first backcrossed with Argopecten irradians to establish a backcrossed family or group, a best hermaphrodite individual is then chosen from the backcrossed family or the backcrossed group with excellent performance, at least three generations of selved families are then bred in succession, and thereby the new marine backcrossedscallop variety which has the characteristics of stable heredity, fast growth, big individual and wide temperature tolerance range is obtained. The new marine backcrossed scallop variety bred by the breeding method has the characteristics of both Argopecten irradians and Argopecten purpuratus, i.e. fast growth, big individual, wide temperature tolerance range and stable heredity, and can be cultured in northern sea areas such as the sea areas of Tsingtao and Dalian, and moreover, the current yield of the new marine backcrossed scallop variety is increased by more than 30 percent in comparisonwith the current yield of Argopecten irradians.

The invention relates to a composite amplification kit for 38 human Y chromosome loci and application thereof. The invention provides a composite amplification system of the 38 human Y chromosome loci. The system includes specific primers for amplifying the 38 loci including 35 STR loci and 3 Indel loci. The 38 pairs of specific primers are subjected to group fluorescence labeling by the six-colorfluorescent labeling technology. Through design and optimization of the primer sequence and the working concentration, simultaneous efficient specific sensitive amplification of the 38 human Y chromosome loci is achieved. A detection result of the composite amplification system has high individual recognition capability and good data compatibility, and can be used in practice for paternity testing and individual recognition, thereby effectively reducing the detection cost of human DNA typing and improving the detection working efficiency.

The invention belongs to the technical field of functional material preparation, and discloses a preparation method and application of a palladium-ion-imprinted composite membrane for selectively separating palladium ions. Specifically, a base film is prepared by using polydimethylsiloxane, and the palladium-ion-imprinted composite membrane can be prepared by use of palladium ion as a template, 8-aminoquinoline and 4-vinylpyridine as ligands, hydroxyethyl methacrylate as a functional monomer, ethylene glycol dimethacrylate a crosslinking agent and azobisisobutyronitrile as an initiator by combination of a sacrificial template method and an ion-imprinting polymerization technique. Selective adsorption experiments are used to study the selective adsorption capacity of the prepared palladium-ion-imprinted composite membrane; selective permeation experiments are used to study the selective permeation capacity of the prepared palladium-ion-imprinted composite membrane for a target substance(palladium ion) and a non-target substance (cobalt ion, copper ion, cadmium ion and nickel ion). The results show that the palladium-ion-imprinted composite membrane prepared by the invention has high specific recognition ability and adsorption separation ability for the palladium ions.

The invention relates to a method for breeding a novel hybrid scallop variety of Argopecten purpuratus and Argopecten irradians irradians. The method comprises the following steps of: establishing a plurality of Argopecten purpuratus and Argopecten irradians irradians hybrid first filial generation families by utilizing the fertilization of eggs of the Argopecten purpuratus and sperms of the Argopecten irradians irradians, selecting excellent hybrid families according to phenotypes of the hybrid families, selecting excellent androgynous individuals from the excellent hybrid families, accelerating maturity, and self-fertilizing respectively to establish a plurality of self-fertilization families; selecting excellent families from the self-fertilization families in the next year, further selecting excellent androgynous individuals from the excellent families and self-fertilizing, and selecting; and continuously self-fertilizing more than three generations by the same method so as to obtain the novel hybrid scallop variety of the Argopecten purpuratus and the Argopecten irradians irradians which has stable inheritance, is large in individuals and wide in a temperature tolerance rangeand can grow quickly. The novel variety can also be realized by a population breeding method. The novel hybrid scallop variety of the Argopecten purpuratus and the Argopecten irradians irradians synchronously has excellent characteristics of the Argopecten irradians irradians and the Argopecten purpuratus, so the novel variety can be cultivated in northern sea areas such as Tsingtao, Dalian and the like; and the yield is improved by over 30 percent compared with that of the Argopecten irradians irradians.

The invention relates to an organic system lithium quinone liquid flow battery. The positive electrode is a carbon material electrode, the negative electrode is a deposition type lithium electrode, and the electrolyte liquid storage tank is filled with electrolyte; the positive electrode electrolyte is quinones and bistrifluorosulfonyl A mixed solution of lithium amide (LiTFSI), the negative electrode electrolyte is LiTFSI solution, and the above solutions all use organic solvents. This type of flow battery has the characteristics of high electrochemical activity, high energy density, and simple assembly process.

The invention discloses a multi-particle silky-feeling scrub cream and a preparation method thereof. The preparation method comprises the following steps: preparing a mixed solution, performing heating and stirring, and sterilizing a finished product. The scrub cream disclosed by the invention has a smooth particle appearance and a silky skin feeling, and the finished product is non-toxic to a human body, cannot scratch a face or influence blood circulation of facial skin during use, has a good scrub effect and a relatively good dirt removal effect. Meanwhile, with mutual combination of a surfactant, an emulsifier and a silky composition, the finished product scrub cream has a relatively wide temperature tolerance range, and the scrub particle composition can be suspended and stabilized at different temperatures, so that the scrub cream is suitable for people with oily skin and oily hair to use, and the dirt removal effect of people with oily skin and oily hair can be improved.

The invention discloses a multifunctional organogel, and a preparation method thereof. The raw materials of the multifunctional organogel comprise a solvent, a monomer, a crosslinking agent, an electrolyte salt, and an initiator; the volume ratio of the solvent to the monomer is controlled to be 1:5-5:1; the molar ratio of the crosslinking agent to the monomer is controlled to be 0.05 to 10%; themolar ratio of the initiator to the monomer is controlled to be 0.1 to 10%; and the concentration of the electrolyte salt in a mixed solution of all the raw materials ranges from 0.1 to 5mol / L. The multifunctional organogel possesses high transparency, high stretching performance, and high conductivity; the temperature resistance and the stability are better than those of conventional hydrogel materials; the temperature resistant range is very wide; the elongation at break is 1219% or higher; and the application prospect in the field of flexible electron and flexible machine is promising.

The invention discloses bacillus subtillis OPUS-001 for producing medium-low-temperature beta-mannase. Preservation number of the bacterial strain in China General Microbiological Culture Collection Center is CGMCC No.7361. The bacterial strain disclosed by the invention is simple in culture method, raw materials are easily available, enzyme yield of the bacterial strain is strong; produced alkali beta-mannase enzyme preparation is high in activity, alkaline-resistant and salt-resistant, has efficient gel breaking capability for oil field guar gum or modified guar gum fracturing fluid under a medium-low region temperature condition, is free of incompatibility with most of chemical assistants in the fracturing fluid, and can satisfy needs of oil field fracturing projects.

The present invention relates to a composite amplification kit of 25 human chromosomal loci and its application. The present invention provides a compound amplification system of 25 loci of human chromosomes, comprising specific primers for amplifying 25 loci, the 25 loci including 23 autosomal STR loci, 1 gender Identify loci and 1 Y chromosome indel site. Using six-color fluorescent labeling technology to group 25 pairs of specific primers for fluorescent labeling, through the design and optimization of primer sequences and working primer concentrations, the simultaneous efficient, specific and sensitive amplification of 25 human chromosomal loci has been achieved. The detection results of the composite amplification system have high individual identification ability and good data compatibility, and can be used for paternity testing and individual identification in practice, effectively reducing the detection cost of human DNA typing and improving the efficiency of human DNA typing. detection efficiency.

The invention involves 44 composite amplification kits and applications of human Y chromosome gene seats.The invention provides a composite amplification system for 44 gene seats in human Y chromosomes, including specific primers used to enlarge 44 gene seats.Chromosome indel.Use the six -color fluorescence markup technology to group the 44 -specific primers, and through the design and optimization of the primer sequence and working concentration, the 44 human Y chromosome gene seats are achieved at the same time as efficient, special, and sensitive.The detection results of the composite amplification system have high individual recognition ability and have good data compatibility, which can be used in practice for parental identification and individual identification, effectively reducing the detection cost of human DNA typing, increasing the improvementThe work efficiency of detection.

The invention aims at providing novel alpha-amylase. The amino acid sequence of the novel alpha-amylase is SEQ ID NO: 1; the genes of the alpha-amylase are encoded, and one nucleotide sequence is SEQ ID NO: 2. The alpha-amylase has a wider temperature tolerance range, the specific activity of the amylase at 50 DEG C is up to 5776 U / mg, and the novel alpha-amylase still can keep about 38% activity at the low temperature of 10 DEG C.

The invention aims at providing novel alpha-amylase. The amino acid sequence of the novel alpha-amylase is SEQ ID NO: 1; the genes of the alpha-amylase are encoded, and one nucleotide sequence is SEQ ID NO: 2. The alpha-amylase has a wider temperature tolerance range, the specific activity of the amylase at 50 DEG C is up to 5776 U / mg, and the novel alpha-amylase still can keep about 38% activity at the low temperature of 10 DEG C.

The invention discloses a composite amplification detection kit for simultaneously detecting 60 autosomes and Y chromosome loci, using polymerase chain reaction to compound and amplify 60 loci, and the amplified products are detected by a gene sequencer . The present invention can detect 60 autosomes and Y chromosome loci at the same time, which is currently the most STR loci detected by capillary electrophoresis in one reaction, and one reaction can simultaneously complete autosome STR and Y chromosome STR library building; the kit has Strong test material adaptability, that is, one kit can amplify the samples of various test materials, and the samples of different test materials include: those extracted by Chelex100 method, magnetic bead extraction method or organic method Male genomic DNA and human male blood or buccal cells collected by any carrier such as filter paper, FTA card, cotton swab, gauze, etc.; this kit has strong amplification specificity and high temperature tolerance.

The invention discloses a Y-STR composite amplification detection kit marked by a fluorescent labeling method and a method for using the same. Multiplex amplification of Y‑STR loci; amplification products are detected by a single-channel or multi-channel capillary gene sequencer; 27 Y‑STR loci include 20 conventional low mutation rate loci and 7 rapid mutation types Gene locus; the fluorescent labeling method is to label the first base at the 5′ end of the primer with a conventional dye, and the sixth base with a short excitation wavelength fluorescent dye to achieve fluorescence energy transfer. The signal intensity generated by this method is higher than that of the conventional method. 2‑10 times higher, you can get more stable and balanced amplification results with higher sensitivity.

The invention discloses a high-strength antifreeze conductive polypyrrole hydrogel and a preparation method thereof, and belongs to the field of polymer materials. The high-strength antifreeze conductive polypyrrole hydrogel is obtained by in-situ chemical oxidative polymerization of a pyrrole monomer in a hydrogen bond-enhanced polymer network, multiple freezing and thawing cycles, and partial displacement of moisture in the gel by a low freezing point medium. The method is wide in raw material source and easy to operate and facilitates industrial production. The high-strength antifreeze conductive polypyrrole hydrogel prepared with the method has good electrical conductivity and mechanical properties, can withstand a relatively wide temperature range, and is unlikely to lose water, shrink and freeze. The high-strength antifreeze conductive polypyrrole hydrogel can withstand subzero 20 DEG C for 72 hours without being frozen, can be placed at 80 DEG C for 4 hours without drying and shrinking, and has an electrical conductivity of 0.5-1.5 S / m, a tensile strength of 0.5 MPa and a compressive elastic modulus of 200 kPa.

The invention discloses the application of a multifunctional organic gel in lithium ion batteries. The raw materials for the preparation of the multifunctional organic gel include: solvents, monomers, crosslinking agents, electrolyte salts and initiators. The solvent The volume ratio of the monomer to the monomer is 1:5 to 5:1; the molar ratio of the crosslinking agent to the monomer is 0.05% to 10%; the molar ratio of the initiator to the monomer is 0.1% to 10%, and the electrolyte The concentration of the salt in the mixed solution of all raw materials is 0.1mol / L-5mol / L. Since the conductivity of the multifunctional organic gel is close to the level of the electrolyte, the present invention uses the multifunctional organic gel for the preparation of lithium-ion batteries, and the batteries can be used normally.