The invention discloses a method of measuring end-to-end delay of mobile phone?video?communication application. Firstly, a section of visual coding timestamp sequence?video is generated and displayed on an LCD display; secondly, a sender mobile phone and a receiver mobile phone build video connection via video?communication application, the visual coding timestamp sequence?video shot by the sender is sent to the receiver, and the receivers displays the conversation content with the visual coding timestamp sequence?video included on a screen; thirdly, a high-definition camera is used for shooting the LCD display and the receiver screen at the same time; and finally, the acquired video data with the visual coding timestamps included is sent to a delay analysis system, the timestamp on each frame is recognized, time difference among the timestamps is calculated, and the end-to-end delay is obtained. According to the method disclosed by the invention, from?the?perspective?of?the?computer vision, the delay measurement problem is converted into the timestamp reorganization problem, the operation process is simple, the difficult problem of synchronization of different clocks is avoided, and accuracy of delay detection is improved.