The invention discloses a vehicle-mounted microphone with a casing adopting bi-color injection molding. The vehicle-mounted microphone with the casing adopting the bi-color injection molding comprises a microphone casing, an electret microphone, a circuit board, a wiring harness and an inserting member. One end of the wiring harness butts against a positive and negative pad on the circuit board, the other end of the wiring harness is inserted in a corresponding groove of the inserting member through a terminal after crimping, and the electret microphone is in welding connection with the circuit board through positive and negative pins. The vehicle-mounted microphone further comprises a dustproof plug, the microphone casing comprises an upper casing and a lower casing, the upper casing and the lower casing comprise an upper casing base, a lower casing base, an upper casing cladding layer and a lower casing cladding layer respectively, the cladding layers and the casing bases are molded into a whole body through injection by means of a bi-color injection molding process, a microphone chamber formed between the upper casing and the lower casing is used for accommodating the electret microphone, and a dustproof chamber is used for accommodating the dustproof plug. The vehicle-mounted microphone with the casing adopting the bi-color injection molding can avoid vibration, protects the microphone, is high in manufacturing efficiency and good in slip resistance and cushion performance, saves cost, and can prevent dust. The casing and internal components are combined stably.