9 results about "Poly-ADP-Ribosylation" patented technology

The invention relates to an artificial nucleic acid molecule comprising at least one 5′ UTR element which is derived from a TOP gene, at least one open reading frame, and preferably at least one histone stem-loop. Optionally the artificial nucleic acid molecule may further comprise, e.g. a poly(A)sequence, a poyladenylation signal, and / or a 3′ UTR. The invention further relates to the use of such an artificial nucleic acid molecule in gene therapy and / or genetic vaccination.

The present invention describes that a pre-mRNA molecule, which temporarily exists before polyadenylation-site induced cleavage occurs, can be targeted by RNase H recruiting antisense oligonucleotides downstream of a transcripts 3' terminus to reduce expression levels of target genes from which the RNA polymerization originates.

The invention provides polynucleotide constructs for the regulation of gene expression by aptamer-based modulation of U1 snRNP-mediated suppression of polyadenylation and methods of using the constructs to regulate gene expression in response to the presence or absence of a ligand that binds the aptamer. The polynucleotide construct contains a U1 binding site in the context of a riboswitch comprising an effector region and an aptamer such that when the aptamer binds a ligand, target gene expression occurs.

The invention belongs to the technical field of biological medicine, and particularly relates to a selective polyadenylation site editing tool and application thereof. The PABPN1 is modified according to the structural domain characteristics of the PABPN1: an effect structural domain CCD structural domain and a phase separation key structural domain EP structural domain are reserved, and an RRM structural domain and other structural domains are removed, so that a minimum unit structural domain with a high-specificity and high-efficiency APA regulation and control function is obtained. According to the present invention, the truncated body protein PABPN1-EP / CCD loses the original RNA binding ability and cannot singly provide the APA regulation function, the PABPN1-EP / CCD is combined with the dPspCas13b protein, the dPspCas13b protein is combined with the crRNA, and the EP / CCD is guided to the target precursor RNA so as to specifically inhibit the target poly (A) site of the target gene;

The invention relates to an artificial nucleic acid molecule comprising at least one 5′UTR element which is derived from a TOP gene, at least one open reading frame, and preferably at least one histone stem-loop. Optionally the artificial nucleic acid molecule may further comprise, e.g. a poly(A)sequence, a poyladenylation signal, and / or a 3′UTR. The invention further relates to the use of such an artificial nucleic acid molecule in gene therapy and / or genetic vaccination.

Provided herein are methods of regulating alternative polyadenylation (APA) of a target RNA in a cell, increasing stability of a target RNA in a cell, preventing degradation of a target RNA in a cell, preventing degradation of a target RNA in a cell, modifying localization of a target RNA in a cell, or increasing synthesis of a protein encoded by a target RNA in a cell, by administering to the cell an RNA regulation unit, wherein the RNA regulation unit comprises an RNA binding protein (RBP) and a gene-targeting agent, wherein the RNA binding protein binds proximal to a poly(A) signal and / or site.

The present invention provides proteins, nucleic acids, systems and methods for visualizing or modulating RNA.