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43 results about "Poly-ADP-Ribosylation" patented technology
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The transfer of multiple ADP-ribose molecules to amino acids within target proteins.
Methods and means for obtaining modified phenotypes
InactiveUS7138565B2Reduce expressionFused cellsOther foreign material introduction processesPoly-A RNAPolyadenylation
Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated target-specific RNA is provided by transcription of a chimeric gene comprising a promoter, a DNA region encoding the target-specific RNA, a self-splicing ribozyme and a DNA region involved in 3′ end formation and polyadenylation.
Owner:COMMONWEALTH SCI & IND RES ORG
Identifying and quantifying small RNAs
One-step RT-PCR methods, compositions and kits for the detection and quantification of small RNAs in a sample are disclosed. The one-step RT-PCR approach involves polyadenylation of a small RNA followed by reverse transcription with a first primer containing a poly(T) sequence and at least two 3′ nucleotides complementary to the 3′ terminal end nucleotides of the small RNA, to produce a cDNA. This may be followed by PCR amplification using the same first primer as the revere primer and a second, forward primer in which a portion of its sequence is complementary to the 3′ terminal end of the cDNA. This may be then followed by detection and / or quantification of the amplified product.
Owner:NORTH CAROLINA STATE UNIV
Kit for auxiliary diagnosis of multiple tumors by taking micro ribonucleic acid (RNA) combination as tumor marker, and detection method of kit
PendingCN105950768AStrong specificityIncreased sensitivityMicrobiological testing/measurementPoly-A RNAIndividualized treatment
The invention relates to a kit for auxiliary diagnosis of multiple tumors by taking a micro ribonucleic acid (RNA) combination as a tumor marker, and a detection method of the kit. The invention belongs to the fields of genetic engineering and oncology. The detection method comprises the steps of (1) extracting RNA from serum; (2) carrying out polyadenylation on the 3' end of micro RNA; (3) purifying and recovering a tailing product; (4) carrying out an inverse transcription reaction on the tailing product; (5) testing the relative expression of the micro RNA by means of a fluorescent quantitative PCR (polymerase chain reaction); (6) analyzing the result of the fluorescent quantitative PCR. The kit has the characteristics of high conservative property, space-time specificity, stability, tissue specificity and the like, and plays an important role in the aspects such as disease pathogenesis research, early diagnosis, individualized treatment and prognosis; furthermore, the kit is simple to operate, convenient in material obtaining, safe and noninvasive, and is conveniently used for screening of a great deal of diseases.
Owner:JIANGSU YINUOWAN CELL CLINIC CO LTD
Methods and means for obtaining modified phenotypes
InactiveUS20050251877A1Reducing phenotypic expressionReduce expressionMicrobiological testing/measurementOther foreign material introduction processesPoly-A RNAPolyadenylation
Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated target-specifc RNA is provided by transcription of a chimeric gene comprising a promoter, a DNA region encoding the target-specific RNA, a self-splicing ribozyme and a DNA region involved in 3′ end formation and polyadenylation.
Owner:COMMONWEALTH SCI & IND RES ORG
Human soluble neuropilin-1 primary polyadenylation signal and uses thereof
InactiveUS20050175591A1Easy to transportImprove translationBiocideGenetic material ingredientsPoly-A RNANucleotide
The human soluble neuropilin-1 (sNRP) polyadenylation signal (sNRP-poly(A)), situated downstream of the GT splice donor site of intron 12 of the full-length neuropilin-1 gene, also functions as the termination codon for sNRP. This 17 nucleotide sequence efficiently facilitates addition of poly(A) tails to RNAs expressed in cells. The present invention shows that this optimally succinct sequence has similar activity to the SV40 polyadenylation signal that is currently used in expression vectors. By using this shorter dual termination / polyadenylation signal and avoiding the need for large and cumbersome polyadenylation signals, expression vectors may be engineered to carry considerably larger genes.
Owner:OREGON HEALTH & SCI UNIV
Ovomucoid promoters and methods of use
The present invention provides novel isolated nucleic acids that comprise an avian nucleic acid sequence comprising a ovomucoid gene expression control region. The ovomucoid promoter region of the present invention will allow expression of an operably linked heterologous nucleic acid insert in a transfected avian cell such as, for example, an oviduct cell. The isolated avian ovomucoid of the present invention may be operably linked with a selected nucleic acid insert, wherein the nucleic acid insert encodes a polypeptide desired to be expressed in a transfected cell. The recombinant DNA of the present invention may further comprise a polyadenylation signal sequence. The present invention further includes expression vectors comprising an isolated avian ovomucoid gene expression control region of the present invention, and transfected cells and transgenic avians comprising the expression vectors.
Owner:SYNAGEVA BIOPHARMA CORP
Multi-gene expression constructs containing modified inteins
InactiveUS20050283850A1Prevent ligation reactionEasy resectionSugar derivativesHydrolasesEucaryotic cellPoly-A RNA
Methods and constructs for the introduction of multiple genes into plants using a single transformation event are described. Constructs contain a single 5′ promoter operably linked to DNA encoding a modified intein splicing unit. The splicing unit is expressed as a polyprotein and consists of a first protein fused to an intein fused to a second protein. The splicing unit has been engineered to promote excision of all non-essential components in the polyprotein but prevent the ligation reactions normally associated with protein splicing. Additional genetic elements encoding inteins and additional proteins can be fused in frame to the 5′-terminus of the coding region for the second protein to form a construct for expression of more than two proteins. A single 3′ termination sequence, such as a polyadenylation sequence when the construct is to be expressed in eucaryotic cells, follows the last coding sequence. These methods and constructs are particularly useful for creating plants with stacked input traits, illustrated by glyphosate tolerant plants producing BT toxin, and / or value added products, illustrated by the production of polyhydroxyalkanoates in plants.
Owner:YIELD10 BIOSCI INC
Transgenic animals with customizable traits
Disclosed are materials and methods for creating customizable traits in animals. In the demonstration of the principle of the subject invention, a keratin-14 specific promoter is used with, red fluorescent protein in the loxp cassette, dominant black (ΔG23) beta defensin 103 in the pigment cassette, and an SV40 (with intron) polyadenylation sequence. When Cre recombinase (or HTNCre) is applied to the animal's skin in a carrier base (e.g., lipid bilayers), fur is permanently genetically modified to turn black in the shape in which it was applied.
Owner:AGGENETICS
Polypeptide human polyadenylation binding protein 20.13 and polynucleotide encoding it
Novel human polyadenylation binding Protein 20.13 and polynucleotide encoding it. The invention also concerns the process of producing the polypeptide by recombinant DNA technique, and methods for treating many diseases e.g. disorder of embryo development, growth developmental disturbant diseases etc. The invention also discloses antagonists against the polypeptide.
Owner:BIOWINDOW GENE DEV INC
Preparation method and application of electrochemical sensor for non-coding RNA
ActiveCN109844514AShorten the timeUniversally applicableMicrobiological testing/measurementMaterial electrochemical variablesPoly-A RNAElectrochemical biosensor
A method for preparing an electrochemical sensor for non-coding RNA, which comprises preparing a sulfonated cup [8] arene-supported reduced graphene oxide by a one-step method, synthesizing a ferroferric oxide nano material by a moist heating method, and adding gold nanoparticles to synthesize Au@SCX8-RGO and Au@Fe3O4 composite materials. After confirming successful material construction by material characterization, a target material is electrochemically detected by a screen printing electrode with toluidine blue and other materials used as electrical signal substances. The electrochemical biosensor prepared by the method can detect the expression quantity of 3'UTR transcripts of non-coding regions of different lengths produced by the selective polyadenylation (APA) phenomenon.
Owner:YUNNAN UNIV
Adenovirus/Alphavirus Hybrid Vector for the Effective Administration and Expression of Therapeutic Genes in Tumour Cells
InactiveUS20070224170A1Guaranteed to workGood antitumor activityBiocideSsRNA viruses positive-sensePoly-A RNAAntigen
The present invention relates to a genic expression adenoviral hybrid vector characterized in that it contains at least the following elements, oriented in the direction 5′ to 3′: i. a first chain of adenoviral origin comprising a first inverted terminal repeat (ITR) sequence and a signal sequence for packaging of the adenovirus; ii. a first non-encoding stuffer sequence; iii. a sequence corresponding to a tissue specific promoter; iv. a chain of cDNA derived from an alphavirus, the sequence of which is partly complementary to an alphaviral RNA sequence, comprising at least a sequence encoding for at least one exogenous gene of interest; v. a polyadenylation sequence; and vi. a second adenoviral inverted terminal repeat (ITR) sequence, it preferably relates to an adenoviral hybrid vector comprising as exogenous gene of interest the therapeutic gene of mammalian interleukin IL-12 and even more preferably human interleukin hIL-12; and to the use of the hybrid vector in a process for transferring genetic material to a cell, particularly a tumor cell that preferably expresses alpha-fetoprotein (AFP), and to its use for inducing an immune response against foreign antigens.
Owner:PROYECTO DE BIOMEDICINA CIMA
Transgenic animals with customizable traits
Disclosed are materials and methods for creating customizable traits in animals. In the demonstration of the principle of the subject invention, a keratin-14 specific promoter is used with red fluorescent protein in the loxp cassette, dominant black ([Delta]G23) beta defensin 103 in the pigment cassette, and an SV40 (with intron) polyadenylation sequence. When Cre recombinase (or HTNCre) is applied to the animal's skin in a carrier base (e.g., lipid bilayers), fur is permanently genetically modified to tum black in the shape in which theHTNCre was applied.
Owner:AGGENETICS
Expression system for cloning toxic genes
Methods of cloning and / or amplifying toxic genes in bacteria using a vector which amplifies the toxic gene in bacteria and also allows subsequent expression in mammalian systems is provided. A vector having an origin of replication, a first promoter, a polylinker, a second promoter in reverse orientation with respect to the first promoter, a poly adenylation signal, and a gene encoding a selectable marker, and optionally an enhancer operably connected to the first promoter, and / or a nucleotide sequence encoding a toxic protein is also provided.
Owner:THE TRUSTEES OF THE UNIV OF PENNSYLVANIA
Hairpin mRNA elements and methods for the regulation of protein translation
InactiveUS10006049B2Stable expressionMinimal protein translationVectorsViral/bacteriophage medical ingredientsPolyadenylationNon-coding RNA
The present invention provides novel mRNA elements capable of forming hairpin, double-stranded RNA structures independent of other non-coding RNAs. These mRNAs are stably expressed, lack polyadenylation tails, and allow minimal protein translation except when in the presence of specific proteins. Also provided, are compositions and kits comprising the mRNA element, as well as methods for its use in the regulation of protein translation. Advantageously, the disclosed elements represent a novel tool useful in regulating the expression of a wide variety of proteins of interest.
Owner:UNIV OF FLORIDA RES FOUNDATION INC
Universal target sequences for siRNA gene silencing
InactiveUS20070123485A1Good treatment effectSugar derivativesGenetic material ingredientsPoly-A RNAGene silencing
The present invention provides methods for designing a sequence for efficient short interference RNA molecules. In particular, the present invention defines a universal target for siRNA derived from the consensus sequence of the polyadenylation signal in conjunction with unique sequences for gene silencing and inhibition of viral replication in a eukaryotic host cell. The present invention further provides methods for the treatment and prevention of diseases and disorders by silencing a gene of a virus, an oncogene, genes encoding transcription factors and many other diseases related genes.
Owner:YISSUM RES DEV CO OF THE HEBREW UNIV OF JERUSALEM LTD
Application of cordycepin in in-vitro maturation culture of oocytes of small pig follicles
ActiveCN103525759AImprove ripening qualityTap reproductive potentialMicroorganism based processesGerm cellsPoly-A RNABiology
The invention relates to application of cordycepin in in-vitro maturation culture of oocytes of small pig follicles, which belongs to the field of auxology of animals. Degrees of polyadenylation of the maternal genes consisting of cyclinB1, C-mos, GDF9, BMP15 and Cdc2 in oocytes determine expression levels of the genes, which has certain promotion effects on the recruitment process where small follicles become dominant follicles and on maturation of oocytes. The expression levels of the genes consisting of C-mos, GDF9 and BMP15 play a leading role in maturation of the oocytes of small follicles. Addition of a 3'-dA inhibitor can improve the expression levels of the genes consisting of C-mos, GDF9 and BMP15, so the maturation rate of the small follicles is increased. The technology of in-vitro maturation culture of oocytes is expected to be popularized and applied in research fields like transgenic technology, nuclear transplantation technology and embryonic stem cell technology.
Owner:JILIN UNIV
Protein Expression
InactiveUS20110195451A1Increase gene expressionEnhance interestBacteriaPeptide/protein ingredientsPromoterDNA
An isolated DNA molecule having a sequence which comprises in a 5′ to 3′ direction (i) one or more promoter elements, (ii) the geneof interest, and (iii) a poly-adenylation 5 signal, and (iv) a terminator element, and expressing the geneof interest incorporated into the DNA molecule in an expression system, and use of said molecule to enhance expression of a gene of interest.
Owner:ISIS INNOVATION LTD
Methods of isolating RNA and mapping of polyadenylation isoforms
InactiveUS20140329700A1Sugar derivativesMicrobiological testing/measurementPoly-A RNAPolyadenylation site
The invention relates to compositions and methods to isolate nucleic acids, and the identification of polyadenylation sites in a gene of interest. In one aspect, the invention provides an oligonucleotide comprising at least one nucleic acid and an affinity moiety, wherein said nucleic acid is 30-60 nucleotides in length and said nucleic acid comprises 1-25 uracil and 5-50 thymine nucleotides.
Owner:RUTGERS THE STATE UNIV
Hepatitis b vaccine and preparation technology thereof
The invention discloses an expression vector expressing a recombinant complete HBV surface antigen comprising L protein, M protein and S protein, prepared by injecting a polynucleotide of a coding region which has a complete HBV envelope gene and a complete 3'-UTR nucleotide containing polyadenylation sites into a pSGM vector identified by the preservation number KCCM 10202.
Owner:DOBEEL CO LTD
Human cytoplasmic polyadenylation element binding protein and uses thereof
InactiveUS20050095632A1Increase in sizeAccelerate the accumulation processSugar derivativesHydrolasesPoly-A RNACytoplasm
The present invention provides genomic and cDNA encoding human cytoplasmic polyadenylation element binding protein, expression vectors comprising human cytoplasmic polyadenylation element binding protein cDNA and host cells that contain the expression vectors. Also provided are recombinant human cytoplasmic polyadenylation element binding protein and polypeptides derived thereof. In addition, the present invention reports that the human Mos 3′ untranslated region (3′ UTR) contains a functional cytoplasmic polyadenylation element (CPE), interacts with the human CPEB1 protein and directs maturation-dependent cytoplasmic polyadenylation of the endogenous Mos mRNA.
Owner:THE BOARD OF TRUSTEES OF THE UNIV OF ARKANSAS
Recombinant Adenovirus Expressing A Gene Encoding Streptolysin O Proetin and Anti-Cancer Composition Comprising Same
A recombinant adenovirus expressing a streptolysin O (SLO) protein comprising a SLO gene; a promoter operably linked to the SLO gene; a polyadenylation signal sequence; and an adenovirus genome lacking E1 gene effectively kills a cancer cell by expressing a pore-forming toxin, SLO protein, and, therefore, is useful for the suicide cancer gene therapy.
Owner:BIOINFRA +1
Pharmaceutical composition for treatment of neurodegenerative diseases or diseases caused by abnormality of RNA binding protein and applications thereof
PendingUS20220110936A1Improve expression levelLower Level RequirementsOrganic active ingredientsNervous disorderPharmaceutical drugSubcellular localization
Disclosed are a pharmaceutical composition for treatment of neurodegenerative diseases or diseases caused by abnormality of RNA binding protein and applications thereof, in particular the application in the treatment of ALS. The pharmaceutical composition can significantly enhance the dynamic performance of stress particles containing RNA binding proteins such as hnRNP A1 and TDP-43 proteins; influences the interaction between the RNA binding proteins and other poly ADP ribosylation modified proteins or other PAR binding proteins; influences the subcellular localization and stress response of RNA binding proteins; influences the liquid-liquid phase separation and aggregation tendency of RNA binding proteins; influences the co-phase separation between RNA binding proteins; influences the interaction of RNA binding proteins in cells; and has a significant inhibitory effect on neurotoxicity caused by RNA binding proteins.
Owner:SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
Method for selecting a high expression recombinant cell line
ActiveCN102365364AShorten the development periodProduce efficientlyNucleic acid vectorFermentationLow densityGene expression
Owner:株式会社斯特利恩
UNIVERSAL TARGET SEQUENCES FOR siRNA GENE SILENCING
The present invention provides a method for the production of a small interference RNA (siRNA) molecules for silencing the expression of a specific gene having AAUAAA as a polyadenylation signal site sequence. The method includes: a) identifying an oligonucleotide sequence of the specific gene, wherein the oligonucleotide sequence is about 15 to about 40 nucleotides in length and comprises (i) the polyadenylation signal site sequence and (ii) unique non-coding sequences flanking the polyadenylation signal site; and b) synthesizing oligonucleotide molecules having the oligonucleotide sequence (a), thereby obtaining an siRNA molecule for silencing the specific gene.
Owner:YISSUM RES DEV CO OF THE HEBREWUNIVERSITY OF JERUSALEM LTD
Cytoplasmic polyadenylation element-binding protein 4 vaccine, and preparation and application thereof
InactiveCN103705920AEasy to prepareGenetic material ingredientsMicroorganism based processesISCOMProtein C
The invention discloses a cytoplasmic polyadenylation element-binding protein 4 vaccine, and preparation and application thereof. The vaccine is a ISCOM-DNA compound, the preparation method of the vaccine comprises: cloning of full-length DNA of cytoplasmic polyadenylation element-binding protein 4, cloning of full-length DNA of tumor necrosis factor BAFF, construction of a recombinant vector pStar-CPEB4 / BAFF, construction of a pStar-CPEB4 / BAFF engineering bacteria, inducible expression of CPEB4, and preparation of the ISCOM-DAN compound. The vaccine has good application prospect on neuroglioma controlling. The vaccine has the advantages of being efficient, low in toxicity, simple in preparation method and the like, and is applicable to medicines for tumour immunotherapy.
Owner:中国人民解放军南京军区福州总医院四七六医院
Linear expression constructs for production of influenza virus particles
InactiveUS20100129399A1Highly economicalShorten the timeSsRNA viruses negative-senseBiocideDiseasePoly-A RNA
The present invention provides a linear expression construct free of any conventional amplification and / or selection sequences comprising an RNA polymerase I (polI) promoter and a polI termination signal, inserted between a RNA polymerase II (polII) promoter and a polyadenylation signal useful for the expression of segments of viral RNA, preferably influenza viruses. The inventive construct is useful for efficient and fast production of viral particles, especially for producing vaccine formulations for the treatment of epidemic and / or pandemic diseases.
Owner:OLOGY BIOSERVICES INC
Methods and compositions for integration-defective lentiviral vectors
The present invention provides an integration-defective lentiviral vector based on a parental lentivirus and related methods, the integration-defective lentiviral vector including one or more of the following: (a) a mutation, deletion or other modification of one or more binding sites for a host factor involved in gene silencing; (b) an addition of one or more binding sites for a transcription activator, which can be natural (such as but not limited to ubiquitous and / or tissue / cell type specific) including but not limited to SP1 NFkB, or synthetic including but not limited to binding sites for tetracycline regulated trans activators tTA, rtTA, tT65, and / or rtT65; (c) one or more nucleic acid sequences from a SV40 genome, wherein the one or more sequences are obtained from a region of the SV40 genome upstream to the SV40 poly-adenylation signal; (d) a shRNA expression cassette, which encodes a shRNA directed to a host gene involved in epigenetic silencing and / or in DNA repair pathways; or (e) any combination of (a), (b), (c) and (d), wherein as compared to the parental lentivirus, the integration-defective lentiviral vector resists gene silencing.
Owner:THE UNIV OF NORTH CAROLINA AT CHAPEL HILL
Methods and compositions for integration defective lentiviral vectors
The present invention provides an integration-defective lentiviral vector based on a parental lentivirus and related methods, the integration-defective lentiviral vector including one or more of the following: (a) a mutation, deletion or other modification of one or more binding sites for a host factor involved in gene silencing; (b) an addition of one or more binding sites for a transcription activator, which can be natural (such as but not limited to ubiquitous and / or tissue / cell type specific) including but not limited to SP1 NFkB, or synthetic including but not limited to binding sites for tetracycline regulated trans activators tTA, rtTA, tT65, and / or rtT65; (c) one or more nucleic acid sequences from a SV40 genome, wherein the one or more sequences are obtained from a region of the SV40 genome upstream to the SV40 poly-adenylation signal; (d) a shRNA expression cassette, which encodes a shRNA directed to a host gene involved in epigenetic silencing and / or in DNA repair pathways; or (e) any combination of (a), (b), (c) and (d), wherein as compared to the parental lentivirus, the integration-defective lentiviral vector resists gene silencing.
Owner:THE UNIV OF NORTH CAROLINA AT CHAPEL HILL
Application of cordycepin in in-vitro maturation culture of oocytes of small pig follicles
ActiveCN103525759BImprove ripening qualityTap reproductive potentialMicroorganism based processesGerm cellsPoly-A RNAPromotion effect
The invention relates to application of cordycepin in in-vitro maturation culture of oocytes of small pig follicles, which belongs to the field of auxology of animals. Degrees of polyadenylation of the maternal genes consisting of cyclinB1, C-mos, GDF9, BMP15 and Cdc2 in oocytes determine expression levels of the genes, which has certain promotion effects on the recruitment process where small follicles become dominant follicles and on maturation of oocytes. The expression levels of the genes consisting of C-mos, GDF9 and BMP15 play a leading role in maturation of the oocytes of small follicles. Addition of a 3'-dA inhibitor can improve the expression levels of the genes consisting of C-mos, GDF9 and BMP15, so the maturation rate of the small follicles is increased. The technology of in-vitro maturation culture of oocytes is expected to be popularized and applied in research fields like transgenic technology, nuclear transplantation technology and embryonic stem cell technology.
Owner:JILIN UNIV
Expression vector for animal cells including csp-b 5'-sar factor and method for producing recombinant proteins using same
ActiveCN103797123AImprove expression rateOvercoming gene expression repressionVectorsEnzymesPoly-A RNABiotechnology
The present invention relates to an expression vector for animal cells, comprising: (a) CSP-B (Cytotoxic Serine Protease-B) 5'-SAR (Scaffold or Matrix Attachment Region); (b) a promoter operable in animal cells; and (c) a polyadenylation sequence, and to a method for producing recombinant proteins using same. The vector of the present invention includes CSP-B 5'-SAR, and thus has the effect of overcoming the inhibition of gene expression according to the position of a foreign gene introduced into an animal cell, and significantly improving the expression rate of a target protein. The vector of the present invention effectively expresses recombinant proteins for drugs or antibodies in animal cells. The vector of the present invention and the method for producing recombinant proteins using same may be very usefully applied to the industrial mass production of drugs.
Owner:CHONG KUN DONG CORP
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