38results about How to "Shorten the development period" patented technology

The present invention relates to a method for selecting a high expression recombinant cell line using an expression vector including: (i) a gene expression cassette containing a selection marker gene to which polyA is inactively connected; and (ii) a gene expression cassette for coding target recombinant proteins to which polyA is actively connected. The method according to the present invention enables the selection of high productivity cell clones from cell populations of 10 times or more less than the cell populations of conventional cell line selection methods. Particularly, the method of the present invention uses low density MTX in selecting high productivity cell clones to shorten the time taken for developing a cell line, as compared to a conventional phased gene amplification strategy which increases the amount of MTX and includes several amplification phases. The method of the present invention achieves an improved efficiency in producing proteins in the event general selection marker genes other than MTX are used.

Disclosed are an equipment control program and an equipment control device in which the amount of the modification of a program in an upper layer associated with the modification of a program in a lower layer can be reduced to suppress an increase in a development period, development cost, or the like when programs for controlling a vehicle-mounted device are hierarchically organized. A program (110) in an application layer (upper layer) receives the input information given from a platform layer (lower layer) and allows an input information abstracting section (113) to abstract the input information. Judgment sections (114), a priority condition judging section (115), and an information processing section (116) perform judgment, information processing, or the like on the basis of the abstracted information. The processing result of the information processing section (116) is outputted after being converted into an information format suitable for the platform layer by an output information converting section (117). In the case where the information format or the like given / received in the application layer and the platform layer is modified, the input information abstracting section (113) and output information converting section (117) of the program (110) in the application layer may be modified.

The present invention discloses an artificial breeding and generation method for thyestillagebleri, and an artificial feedstuff for the thyestillagebleri larvae. The method comprises the following steps: placing a single mature thyestillagebleri larva in a test tube at a temperature of 20-30 DEG C and relative humidity of 60-80%; after pupating and emerging, carrying out pairing for the imago, then placing the paired imagoes in a container provided with piemarker stems to carry out copulation and oviposition; carrying out disinfection for eggs; placing the hatched larvae in a container filledwith the artificial feedstuff to breed until the larvae mature, wherein the artificial feedstuff in the container is replaced for a plurality of times. A formula of the artificial feedstuff comprises, by weight, 5-20% of corn flour, 5-20% of soybean powder, 3-10% of maggot powder, 3-10% of sugar, 3-7% of agar, 0.1-1% of Vc, 0.1-1% of a preservative and 50-70% of water. According to the present invention, the thyestillagebleri is bred through the method provided by the present invention, the diapause of the larvae is not generated, the developmental duration is substantially shortened; the feedstuff does not contain the host component; the main components are easy to obtain; the production method has characteristics of simple process, low cost and no seasonal restriction, and is applicablefor large-scale indoor breeding.

The invention relates to a controller for an internal combustion engine which can simplify a control operation while achieving highly functional control through proper arbitration of a plurality of requests. The controller includes an emission control section (52) to output each request based on individual purposes to an internal combustion engine; a fuel consumption control section (54); an idlestability control section (56), an efficiency arbitration section (58) to arbitrate requests from those sections; and an actuator instruction value calculation section (60) to determine the instruction values given to a plurality of actuators provided on the internal combustion engine in accordance with a result of arbitration performed by the efficiency arbitration section (58). The requests arethose for the torque efficiency indicative of a ratio of a requested torque to a reference torque obtained when operating points of a plurality of actuators are optimized.

The invention discloses a method for a large number of breeding of Lepidos wasps based on yacon as a host plant, and relates to a new method for breeding Lepidos wasps, which uses yacon as an intermediate host plant and whitefly in a greenhouse as a host to carry out Industrial mass breeding of Aphid wasps: (1) Cultivation of healthy yacon plants; (2) Greenhouse whitefly seedling breeding; (3) Greenhouse whitefly host nymphs mass breeding; (4) Greenhouse whitefly seedling production ; (5) Aphid wasps breed in large numbers. The present invention uses yacon as an intermediate host plant to breed a large number of Lepidos wasps, shortens the breeding cycle of greenhouse whitefly and Lepidos wasps, further improves the quality of large-scale breeding of Lepidos wasps, and is suitable for industrial breeding of Lepidos wasps have a positive meaning. The breeding cycle of whitefly and wasps further improves the mass breeding quality of wasps, which is of positive significance to the industrial breeding of wasps.

A program of an application layer (upper layer) receives input information supplied from a platform layer (lower layer) and abstracts the information by an input information abstraction part. Based on the abstracted information, determination parts, a prioritized condition determination section and an information processing section make determinations and perform information processing, for example. A processing result obtained by the information processing section is converted into an information format suitable for the platform layer by an output information conversion section, and is outputted therefrom. When a format of information exchanged between the application layer and platform layer, for example, is changed, changes may be made to the input information abstraction part and the output information conversion section of the program of the application layer.