The invention discloses a longitude and latitude detection device for glass fiber cloth research and development. The longitude and latitude detection device comprises a supporting mechanism, a counting mechanism and an observation mechanism. The supporting mechanism comprises a bottom plate, an anti-skid assembly and a sliding block, a through hole is formed in the middle of the bottom plate andis a rectangular through hole, sliding grooves are formed in the two sides of the upper surface of the bottom plate, the sections of the sliding grooves are of T-shaped structures, the anti-skid assembly is fixedly connected to the lower surface of the bottom plate, and sliding blocks are arranged in the sliding grooves in a sliding mode; and the counting mechanism comprises a supporting plate, alimiting rod, a rotary knob assembly, a rotating rod, a moving block and a counting needle, the supporting plate is fixedly connected to the upper sides of the sliding blocks, the rotary knob assemblyis arranged on one side of the supporting plate, and one end of the rotating rod is fixedly connected to one side of the rotary knob assembly. According to the scheme, multiple groups of parallel data can be detected under the condition that the whole device is not moved, and the quality of the glass fiber cloth can be detected more accurately through comparison.