The invention provides multi-response fluorescent ink and a preparation method. The multi-response fluorescent ink comprises, by weight, 0.5-5 parts of rare earth metal salt, 1-5 parts of organic ligands, 50-70 parts of organic solvents, 20-30 parts of polymer resin and 0.1-2 parts of auxiliaries, wherein the rare earth metal salt is selected from one or two of europium salt and terbium salt, theorganic ligands are beta-diketone molecules, the polymer resin is selected from one or more of acrylic resin, epoxy resin, ethylene resin and polyurethane, and the auxiliaries are selected from dryingcatalysts, diluents, viscosity reducers, thinners, antioxidants, anti-gelling agents, defoaming agents, surfactants, anti-pitting agents, slipping agents and plasticizers. The fluorescent ink presents different fluorescent colors under the ultraviolet condition of different wavelengths, can present multicolor fluorescence after being stimulated by different substances, and has the advantages of high anti-counterfeiting level and strong anti-counterfeiting capacity.