The invention relates to a multi-stage electrochemical hydrogen pump for preparing high-pressure hydrogen. The multi-stage electrochemical hydrogen pump for preparing the high-pressure hydrogen is composed of a fastening end plate, a fastening screw rod, an insulating plate, an electrode plate, a separation fixing plate, a gas-liquid transmission plate, a membrane electrode assembly and the like.The separation fixing plate and the gas-liquid transmission plate form a gas chamber assembly, the inner hole space of the gas chamber assembly directly serves as a gas chamber, the dead volume is reduced, and the safety is improved; the contact area with the membrane electrode is increased, the best support is provided for the membrane electrode, meanwhile, the ohmic impedance is reduced, and theefficiency of the hydrogen pump is improved; the multi-stage electrochemical hydrogen pump is formed by connecting a plurality of hydrogen pumps in series, and step-by-step pressurization is achieved. The structure is simple, machining and manufacturing are easy, large-scale production is facilitated, the cost can be greatly reduced, and meanwhile the assembling procedure and requirements are relatively simple and easy to control. The technology is wide in application range, can be used for small portable mobile hydrogen supercharging equipment, and a large fixed hydrogen refueling station and the like, and can also be used for high-purity hydrogen purification equipment.