Relating to the technical field of edible fungus cultivation, the invention provides a culture method of selenium-rich edible fungus. The method includes the steps of: (1) blending: taking chicken manure, duck manure, pig manure, selenium-rich cob, wheat bran, bean dregs, humic acid, bone meal, volcanic cinder, lotus seed skin, garlic, mangosteen skin, Lycium ruthenicum residue, selenium-rich waste tea, expired milk and purple sweet potato, conducting fermentation to prepare a culture material, adjusting the moisture content of the culture material to 60-70%, and adjusting the pH value of the culture material to 5.3-6.3; (2) bagging; (3) sterilization; (4) inoculation; (5) mycelium culture; (6) fungus yield management; and (7) harvesting. The culture method of selenium-rich edible fungus provided by the invention can produce selenium-rich edible fungus with high yield.