The invention discloses a micro light-emitting 
diode, a display substrate, a manufacturing method of the display substrate and a 
display device, and belongs to the technical field of display. The micro light-emitting 
diode at least comprises a first 
electrode, a second 
electrode, a first 
semiconductor part, a light-emitting part and a second 
semiconductor part, the light-emitting part is located between the first 
semiconductor part and the second semiconductor part, the first 
electrode is located on the side, away from the light-emitting part, of the first semiconductor part, and the second electrode is located on the side, away from the light-emitting part, of the second semiconductor part. The 
orthographic projection of the first electrode on the first semiconductor part at least covers 50% of the area of the first semiconductor part. The display substrate comprises a plurality of micro light-emitting diodes. The manufacturing method of the display substrate is used for manufacturing the display substrate. The 
display device comprises the display substrate. According to the micro light-emitting 
diode provided by the invention, the alignment difficulty during transfer can be reduced, and the yield of a 
die bonding process is improved, so that the light-emitting efficiency is improved.