The invention provides an array substrate, a preparing method thereof and a display device, wherein the preparing method comprises the steps of: forming an active material layer, a gate electrode insulating layer and a metal film on a substrate, forming a pattern which comprises an active layer and a pattern that comprises a gate electrode, a source electrode, a drain electrode, a gate line and a data line through a primary composition process; forming a passivation layer on the substrate, forming a source electrode contact hole, a drain electrode contact hole and an over-bridge structure contact hole through a secondary composition process; and forming a transparent conductive film on the substrate, eliminating partial transparent conductive film through a film peeling process for forming a source electrode contact part, a drain electrode contact hole, a pixel electrode and an over-bridge structure. According to the preparing method, number of times in using the composition process is reduced. The array substrate has advantages of simple preparing process, high production efficiency and high yield rate.