The invention discloses a vacuum gate valve for a mass spectrometer. The vacuum gate valve comprises a valve seat and a valve element, and a sealing ring is arranged between the valve seat and the valve element. The valve element is provided with a squeezing mechanism used for enabling the valve element to tightly press the sealing ring. The invention further discloses a mass spectrometry instrument, the mass spectrometry instrument comprises an ion source cavity and an analysis chamber which is communicated with the ion source cavity, and a communicated channel of the ion source cavity and the analysis chamber is provided with the vacuum gate valve. In addition, the invention discloses a method for establishing a high vacuum environment in mass spectrometry sample change. The method includes the steps that the communicated channel of the ion source cavity and the analysis chamber is closed through the vacuum gate valve, the ion source cavity is opened for changing samples, after the samples are changed, the ion source cavity is pre-vacuumized, the vacuum gate valve is opened so that the ion source cavity and the analysis chamfer can be communicated with each other, and vacuumizing is conducted. According to the devices and the method, the vacuum needed by detection of the mass spectrometer can be established quickly after the samples are changed, the sealing effect is good, and the sample changing efficiency is high.