The utility model provides an up-and-down bidirectional press-fit guide fine positioning mechanism, which comprises a support and a carrier for placing a product, a positioning plate for positioning and mounting the product is arranged on the support, a first power device for driving the carrier to move towards the positioning plate is mounted on the support, a mounting groove is arranged on the positioning plate, and a second power device for driving the carrier to move towards the mounting groove is arranged on the mounting groove. The mounting groove is a through groove, a first guide slope is arranged on the side, close to the carrier, of the mounting groove, and a second guide slope is arranged on the other side of the mounting groove. The PCBA board is guided and positioned while the shell is precisely positioned through the positioning plate, the function of positioning an upper material and a lower material at the same time is achieved, the upper material and the lower material are positioned through the positioning plate, the positioning precision is 0.03 mm, and the positioning precision is high; the problem that the positioning precision is low when two materials are assembled can be solved, the synchronous positioning function of the two materials is achieved through the structure, and the structure is superior to a common manual independent positioning structure for a single material in the prior art.