The invention provides an energy-saving and environment-friendly bacteria stick preparing method, and relates to the technical field of edible fungi production. The energy-saving and environment-friendly bacteria stick preparing method comprises the following steps of S1, primordium mixing, wherein raw material dried powder is mixed first, and then water is added for adjusting the water content; S2, ozone sterilization, wherein sterilization is conducted with ozone water; S3,adjustment of the humidity ratio of primordia; S4, mother strain mixing, wherein the second generation of strains and potassium ozonide are added into the primordia; S5, stick preparation; S6, spawn running; S7, generation of the finished product. According to the bacteria stick preparing method, the formula of the adopted primordium raw materials is reasonable, the nitrogen source is abundant, nutrients required for the growth of edible fungi are ensured, the growth of the edible fungi is promoted, carbon dioxideis prevented from being accumulated in the preparation process, sufficient oxygen is provided for the edible fungi, the preparation process is energy-saving and environmentally friendly, thorough sterilization is achieved, strain mixing is uniform, the spawn running time is short, and the edible fungi produced by using the bacteria sticks are high in yield.