The invention discloses a printing process of a light guide plate. The printing process of the light guide plate includes the following steps that (1) a light transmission plate is provided; (2) a photo-sensitive glue layer is pasted to a frame of a silk screen, screen dots are designed, the photo-sensitive glue layer is dried with hot air at the temperature of 75-85 DEG C for 10-20 minutes, a film base of a photo-sensitive film is uncovered, then plate burning is carried out, and a screen printing plate is obtained after development; (3) a protection film on the bottom face of the light transmission plate is torn, the surface of the light transmission plate is wiped thoroughly with ethyl alcohol so as to remove residues on the surface, the bottom face is attached to the screen printing plate, halogen-free ink is poured to one end of the screen printing plate, the angle of inclination of a scraping and printing scraper blade is adjusted to be 65-85 degrees, all the ink on the screen printing plate moves towards the other end, and accordingly multiple scattering screen dots are printed; and (4) the bottom face, where the scattering screen dots are printed, of the light transmission plate is irradiated by ultraviolet light for 2-6 seconds, and the light transmission plate is conveyed to a tunnel furnace through a roller bed, is heated at the temperature of 85-95 DEG C for 2-4 minutes to be cured, and is cooled to the temperature of 70-80 DEG C to be thoroughly cured. The scattering screen dots printed through the printing process have the dual effects of light refraction and high light reflection, and have excellent brightness.