The invention discloses an on-chip reflection type quantum amplifier with a strong isolation function. The quantum amplifier provided by the embodiment of the invention is a two-port on-chip device with a strong isolation function, a circulation function and an amplification function, is small in size and low in dissipation, and has nonreciprocity. According to the on-chip quantum amplifier with the strong isolation function, the gain bandwidth product of the amplifier is increased, the strong isolation function is achieved, meanwhile, an HEMT can be replaced, and the cost of a superconducting quantum computer and the cost of a test system of the superconducting quantum computer are greatly reduced. The on-chip quantum amplifier with the strong isolation function, provided by the embodiment of the invention, can be directly connected with the quantum chip, so that the quantum chip is protected from counteractive interference of a subsequent circuit. Based on the on-chip quantum amplifier provided by the embodiment of the invention, not only is the superconducting quantum test system using the on-chip quantum amplifier provided by the embodiment of the invention simplified, but also the size of the superconducting quantum test system is remarkably reduced, and large-scale development of superconducting quantum computers is facilitated.