The invention provides a punching and pesticide injection device for disease and pest control over fruit trees. The device comprises a supporting shell, a motor, a drill bit, a micro air pump, a firstspeed control valve, a three-way valve, a check valve, a second speed control valve, a first connector, a second connector and a piston, wherein the piston is installed in a pesticide injector, the motor is installed on the left end face of the supporting shell, the drill bit is installed on the left end face of the motor, and the micro air pump is installed on the upper end face of the supporting shell; the first speed control valve is installed on the left side of the micro air pump, the three-way valve is installed on the left side of the first speed control valve, the check valve is installed on the upper side of the three-way valve, and the second speed control valve is installed on the upper end face of the check valve; the first connector is installed on the annular side face of awater outlet column, and the second connector is installed on the upper end face of the drill bit. Due to the design, the problem is solved that joints of an original punching and pesticide injectiondevice for disease and pest control over the fruit trees easily become loose to cause pesticide waste, the structure is reasonable, combined installation is facilitated, the cleaning effect is good, and the practicability is high.