The invention provides aquaculture apparatus with the partition culture function. The aquaculture apparatus comprises a support frame, a lower support frame, an upper support frame, a protection net cage, support rods, an installation platform, connecting rods, a material outlet box, a protection cabinet, a PLC, a drive switch, an air pipe, positioning seats, a movable partition frame structure, an auxiliary sprinkling tray structure, a power supply protection box structure, an air pump and a flap valve, wherein the support frame is installed around the lower support frame and the upper support frame through bolts, the protection net cage is embedded around the inner walls of the support frame, the lower support frame and the upper support frame, the support rods are connected with the left and right sides of the upper part of the upper support frame through bolts, and the installation platform is integrally arranged at the upper ends of the support rods. The beneficial effect of the aquaculture apparatus is that the movable partition frame structure is arranged to facilitate activities in cooperation with a partition net between a first movable cylinder and a second movable cylinder, thereby the partition work is performed, and functionality is increased.