The invention discloses a skylight positioning station which comprises a working platform, a lifting mechanism, an X-Y adjusting assembly, a rotating assembly, a taking assembly and a clamping mechanism. The lifting mechanism is arranged in the upper center of the working platform, the X-Y adjusting assembly is arranged on the lifting platform, the rotating assembly and the taking assembly are arranged on the X-Y adjusting assembly, the clamping mechanism is arranged on the working platform and comprises two moving guide rails, a sliding block, two moving assemblies, a first clamping assembly,a second clamping assembly, a third clamping assembly and two jacking assemblies, wherein the two moving guide rails are arranged on the two sides of the upper end face of the working platform in parallel, and the two moving assemblies are oppositely arranged on the two sides of the upper portion of the working platform and each comprise two moving supports, a driving air cylinder and an installation plate. The position of a skylight on the positioning station is accurate and unique, alignment for a mechanical hand is stable and reliable, automatic positioning for the skylight and taking positioning for the mechanical hand can be successfully completed, and high-quality products are provided for automatic plants.