The invention relates to a liquid surface tension testing method based on mechanical vibration of a liquid droplet. The method specifically comprises the following steps: placing a hydrophobic substrate on a Doppler ultrasonic probe, and generating a liquid droplet on the hydrophobic substrate; vibrating a triggering device to freely vibrate the liquid droplet; converting free vibration of capillary waves on the surface of the liquid droplet into an ultrasonic Doppler frequency shift signal through the ultrasonic probe by using a non-focusing continuous wave Doppler method, and amplifying theultrasonic Doppler frequency shift signal; acquiring a free vibration characteristic diagram of the liquid droplet from the ultrasonic Doppler frequency shift signal by using a computer signal processing module, performing Fourier transform so as to obtain a vibration frequency characteristic diagram, analyzing the vibration frequency characteristic diagram, and reading frequency of a second harmonic generation peak; by taking the reciprocal of the mass of the liquid droplet as a transverse coordinate, and the square of the frequency of the second harmonic generation peak as a longitudinal coordinate, drawing diagrams, fitting straight lines, calculating a straight slope, and further calculating the surface tension of the liquid droplet. The method based on ultrasonic Doppler frequency shifting is capable of carrying out frequency measurement on ultrasonic signals, and has the advantages of reagent conservation, high performance cost ratios and portability.