56 results about "Neural immune" patented technology

The invention provides a micro-fluidic chip, a model for realizing accurate positioning of an organoid and sequential interaction of a tumor microenvironment, and a construction method of the model. The micro-fluidic chip comprises a chip main body and a chip bottom plate, a blood vessel channel, a central channel, a neural immune cell channel and a culture medium channel are sequentially arranged on the lower surface of the chip main body; the chip bottom plate is arranged on the lower surface of the chip main body and seals each channel; one side of the blood vessel channel is communicated with one side of the central channel, the other side of the central channel is communicated with one side of the neural immune cell channel, and the other side of the neural immune cell channel is communicated with one side of the culture medium channel; the upper surface of the chip main body is provided with an open hole, and the open hole penetrates through the chip main body and is communicated with the middle position of the central channel. According to the model manufactured by the micro-fluidic chip, accurate positioning of tumor organs can be realized through combination of an automatic positioning device and physical constraint, and multi-dimensional cell interaction is supported by dynamic environment regulation and control.

The invention provides multifunctional bionic hydrogel as well as preparation and application thereof in traumatic brain injury treatment, and belongs to the field of biomedical materials. The preparation method comprises the following steps: combining polydopamine modified hemoglobin nanoparticles with curcumin-based carbon quantum dots with anti-inflammatory activity, and embedding into bionic hyaluronic acid-collagen double-network hydrogel, so as to prepare the multifunctional bionic hydrogel. The hydrogel can effectively relieve neuron hypoxia, reduce apoptosis, promote the polarity of microglial cells to be converted into anti-inflammatory phenotype, and significantly down-regulate the expression of S100A8, thereby inhibiting pathological neural immune crosstalk. In a traumatic brain injury model, hydrogel implantation improves the pathological microenvironment, enhances endogenous nerve regeneration, and ultimately results in significant neurological function and cognitive recovery. The multifunctional bionic hydrogel prepared by the invention has a good application prospect in preparation of materials for preventing and / or treating traumatic brain injury.

The preparation method comprises the following steps: by taking an oriented polylactic acid nanofiber membrane as a raw material, carrying out homogenization treatment to obtain polylactic acid piezoelectric short fibers; gelMA is used as a skeleton matrix, polylactic acid short fibers, LAP and DRG cells are compounded, a 0 / 90-degree alternate grid structure scaffold is constructed through pneumatic extrusion type biological printing, and the dual-network piezoelectric biological scaffold is formed through blue light crosslinking curing. The piezoelectric effect of polylactic acid short fibers is activated through ultrasonic stimulation, mechanical energy is converted into local electric signals, loaded DRG cells are directly driven to secrete CGRP in situ, macrophages are dynamically regulated and controlled to be polarized to M2 anti-inflammatory phenotypes, nerve-immune microenvironment disorder of diabetic bone defects is corrected, vascularization and bone regeneration are promoted, and the diabetic bone defect treatment effect is improved. The problems of short delivery half-life period of traditional cell factors, difficulty in nerve function reconstruction and chronic inflammation caused by non-degradable piezoelectric materials are solved, and non-invasive and degradable precise bone repair is realized.

The invention provides an immersive virtual patient diagnosis and treatment thinking training system for neural immune diseases. The immersive virtual patient diagnosis and treatment thinking training system comprises a multi-dimensional case holographic modeling module, a diagnosis and treatment thinking anchor point dynamic capturing module, a double-AI collaborative diagnosis and treatment deduction module, an evidence-based feedback and thinking correction module and a diagnosis and treatment capability quantitative evaluation module which are in data connection in sequence and form closed-loop optimization. The objective of the invention is to solve the pain points of scene staticization, thinking assessment deficiency, clinical separation of an AI scheme and no closed-loop optimization in existing neural immune disease diagnosis and treatment training.

This invention provides the application of triptolide in the preparation of drugs for treating microglial inflammation-related neuroimmunological disorders. Experiments show that triptolide significantly inhibits the expression, migration, phagocytosis, and morphological activation of microglial inflammatory factors, and exerts an anti-microglial inflammatory effect in animal models. Through DARTS technology combined with siRNA screening, ACOX1 was identified as its key target, and the direct binding between the two was verified by CETSA, SPR, and molecular docking.

The application provides application of dimethyl itaconate in prevention and treatment of chronic stress-induced anxiety. The application finds that dimethyl itaconate can inhibit excessive activation of microglial cells in a stress state, improve mitochondrial structure and function abnormalities of the microglial cells, and reduce expression of a lysosome marker CD68, so as to inhibit central nervous inflammation, maintain central nervous immune homeostasis, prevent and relieve anxiety-like behavior induced by chronic stress, and effectively prevent and relieve occurrence and development of chronic stress-induced anxiety (generalized anxiety disorder).

The application belongs to the field of stem cell biology and relates to a culture method of a 3D nerve immune organoid containing microglia cells, which comprises the following steps: S1, inducing induced pluripotent stem cells in ectoderm direction and mesoderm direction respectively to obtain nerve-like embryoid bodies and yolk sac-like embryoid bodies; S2, culturing the nerve-like embryoid bodies to make them differentiate into nerves to obtain nerve ring structures, re-digesting the nerve ring structures to obtain nerve progenitor cell single cells, and culturing the yolk sac-like embryoid bodies to make them generate macrophage progenitor cells; and S3, fusing the nerve progenitor cell single cells and the macrophage progenitor cells and continuing to culture to form the 3D nerve immune organoid containing microglia cells. The brain-like organoid of the application can realize the following functions: exploring the control of microglia cells on the proportion of progenitor cells in the development stage, the change of phagocytosis, the control on the number of mature neurons, the influence on cell proliferation and apoptosis, and simultaneously accepting external stimulation and making corresponding functional stress changes.

Methods and systems for modulating neural immune responses while monitoring biomarkers that may be directly or indirectly associated with neural immune function. Neural modulation therapy may be delivered and biomarker levels may be determined. Some biomarker levels are determined from bodily fluids collected from the patient and compared to predetermined threshold levels. Therapeutic stimulation is delivered to a patient using one or more electrode leads and / or optical fibers.

The invention relates to application of NADH (nicotinamide adenine dinucleotide) in preparation of drugs for preventing or treating autism, and further provides a liposome nanoparticle oral preparation for preventing or treating autism, the NADH can reduce central nervous system inflammation, and tests prove that the NADH can remarkably reduce neural immune inflammation of autism model rats, and can be used for preventing or treating autism. The method has the advantages that the method is simple and convenient to operate and has neuroprotection and progeny rat behavioral correction capacity, social experiment defects of progeny rats are overcome, the engraving behaviors of the progeny rats are reduced, the influence of autism-related behaviors of the progeny rats is reversed, and a new scheme is provided for prevention or treatment of autism.

The invention relates to the fields of biotechnology, traditional Chinese medicines and nutritional health-care products, in particular to a bifidobacterium breve-containing medicinal and edible composite composition and application thereof in preparation of products for preventing or treating anxiety and depression, improving sleep disorders and assisting in weight management. The composite composition is prepared from bifidobacterium breve and a medicinal and edible composition, and the ratio of the bifidobacterium breve to the medicinal and edible composition is 1 * 10 < 10 > CFU of the bifidobacterium breve and 60 g of the medicinal and edible composition. The composition disclosed by the invention has a remarkable synergistic effect, can effectively prevent or relieve anxiety and depression symptoms, can improve sleep quality, and can assist in reducing body weight and body fat. The action mechanism relates to double targets of'intestine-brain-metabolic axis', and the double targets supplement each other to synergistically promote nerve-immunity-endocrine and metabolic balance. The composition disclosed by the invention can be used for preparing medicines, functional foods or health-care products, and provides a safe and effective brand-new choice for intervention of multiple health problems (emotion, sleep and metabolic disorders).

The invention relates to the technical field of biological medicine, and particularly discloses application of a cGAS-STING pathway inhibitor in relieving neural immune response induced by AAV, and the technical key points are as follows: it is found that high-dose AAV can activate a cGAS-STING signal pathway in a nervous system, resulting in inflammatory response, glial cell activation, T cell infiltration and neuron damage; the cGAS-STING pathway is inhibited by means of gene knockout (Stin < + / ->), pharmacological inhibition (such as STING inhibitor H-151) or virus-mediated gene knockout (shSTING) and the like, so that the AAV-induced immune response and neurotoxicity can be effectively reduced, and meanwhile, the expression of a transgenic product carried by the AAV vector is improved. The scheme provided by the invention provides a new strategy and means for optimizing the safety-effectiveness balance of AAV gene therapy.

Devices and system configured to deliver ultrasound to the brain may enhance brain waste clearance for a variety of purposes for acute and / or chronic indications. During use, ultrasound may be projected into multiple regions of the brain to increase circulation of cerebrospinal fluid (CSF) and / or interstitial fluid (ISF) within the brain, increase mechanical mixing of CSF and / or ISF in the skull and / or between intracranial fluid compartments, induce mechanosensitive channel activation, and / or shift a microglial phenotype from the diseaseassociated state to the hemeostatic state, which may impact a neuroimmunologic state of the subject's brain. The ultrasound may be projected into the subject's brain in accordance with an ultrasonic cerebrospinal fluid clearance (UCC) treatment protocol that sets parameters for the UCC treatment. On some occasions, the parameters of a UCC treatment protocol may be responsive to subject characteristics and / or may be used to predict an outcome of treatment.

This invention discloses an animal model of trigeminal neuralgia, its construction method, and its applications. This invention successfully constructed an animal model of trigeminal neuralgia and, through behavioral, cellular, and molecular biological experiments, conducted multi-dimensional research to systematically elucidate the association between changes in macrophages and neurons mediated by the NPY-Y2R signaling pathway and trigeminal neuralgia in the animal model. This model can be used for neuroimmunotherapy of trigeminal neuralgia and enables more effective and precise personalized clinical targeted therapy for patients with trigeminal neuralgia.

This invention discloses a method for preparing and applying an AIE probe for long-term two-photon imaging of the cerebrovascular system in awake mice. The probe is prepared by assembling an AIE precursor with DSPE-mPEG2000-Mal into nanoparticles, followed by coupling with the cell-penetrating peptide TAT. This probe exhibits excellent photostability, long-term vascular retention, good biocompatibility, and neuroimmunocompatibility. In awake mouse models, it can achieve three-dimensional imaging of cerebrovascular systems at depths exceeding 500 micrometers and stably labels blood vessels for up to 35 days without activating microglia. This probe supports multi-scale imaging from acute hemodynamics to long-term structural remodeling, providing a powerful tool for neurovascular research.

The invention belongs to the technical field of cell culture devices, and particularly relates to a device for in-vitro construction of a 3D neural immune cell co-culture model.The device comprises a vessel body container, a vessel cover is arranged outside the vessel body container, a biological scaffold is arranged in the vessel body container, and the biological scaffold comprises a static column, a plurality of movable plates and a plurality of half grooves; the plurality of movable plates are arranged around the static column and can rotate by taking the static column as an axis, the plurality of movable plates divide the vessel body container into a plurality of culture chambers, and each movable plate is provided with a plurality of gaps, so that the plurality of culture chambers are communicated through the gaps; half grooves are formed in the opposite positions of every two adjacent movable plates; and when two adjacent movable plates are folded, the corresponding half grooves are spliced to form a complete cell inoculation groove. The device is good in stability, different culture chambers are arranged in the vessel body container, materials cannot be leaked to the outside of the vessel body container, pollution is prevented, and safety is high.

The invention discloses an MXene (at) SA fiber electrode capable of reducing neural immune response as well as a preparation method and application of the MXene (at) SA fiber electrode. The preparation method comprises the following steps: step 1, concentrating a single-layer MXene dispersion liquid into MXene spinning slurry with the concentration of 90-110 mg / mL; 2, respectively injecting the MXene spinning slurry and the sodium alginate solution into an outer needle and an inner needle of a coaxial needle head device of a wet spinning machine, extruding, and enabling the MXene spinning slurry and the sodium alginate solution to sequentially pass through a 90-degree bent needle head and a polytetrafluoroethylene tube to enter a rotary coagulating bath to form MXene-coated SA fibers; and step 3, washing with an ethanol / aqueous solution, collecting, and drying to obtain the MXene (at) SA fiber electrode. The MXene (at) SA fiber electrode is used as a brain implantation electrode, neuroinflammatory response generated during implantation can be effectively reduced, the problem of tissue-electrode mismatching is avoided, cell behavior regulation is enhanced, and integration of neural electrodes is improved.

The present application relates to a soothing repair composition containing Saintgrass nuclease and its application in sensitive skin care, belonging to the technical field of cosmetics. The soothing repair composition containing Saintgrass nuclease includes Saintgrass nuclease, chamomile extract, Sophora flavescens root extract and Magnolia kobus extract, which synergistically act on multiple links such as nerves, immunity and inflammation to comprehensively soothe sensitive symptoms, repair skin barrier, and the effect is significantly better than that of single component, is safe and non-irritating, and has a wide application prospect in the field of skin care.

The invention relates to a soothing anti-inflammatory composition suitable for sensitive skin and a preparation method of the soothing anti-inflammatory composition, and belongs to the technical field of cosmetics. The soothing and anti-inflammatory composition comprises the following components: vetiver extract, European basswood extract, peucedanum decursum extract and European chestnut leaf extract, wherein the weight ratio of the vetiver extract to the European basswood extract to the peucedanum decursum extract to the European chestnut leaf extract is (1-3): (2-5): (1-4): (2-6). The four extracts form a multi-dimensional collaborative network, and a comprehensive, mild and effective soothing, bacteriostatic and anti-inflammatory scheme from nerve-immunity-microorganism-physical barrier is provided for sensitive skin users.

The invention belongs to the technical field of biology, and relates to a construction method of a gene modification animal model, in particular to a construction method of a Cd47 knockout rat model and verification of the knockout effect of the Cd47 knockout rat model. The invention provides a construction method of a Cd47 knockout rat model, which comprises the following steps: constructing the Cd47 knockout rat model based on a CRISPR / Cas9 system by utilizing a screened high-activity gRNA target site; the inventor identifies the gene knockout and protein expression conditions and growth conditions of the model, the growth and development conditions of immune organs and the phenotypes of red blood cells and T cells; the model is proved to realize the knockout of each homologous isomer of the endogenous Cd47 gene of the rat, and can be applied to the fields of related researches of the CD47 protein in nerves, immunity and tumors and the like.

The invention provides a soothing composition for improving skin tolerance and application of the soothing composition. The composition is prepared from schizandrin A, dihydroquercetin, jonquil glucoside and panthenol. The raw materials in the composition cooperate with each other to synchronously intervene four major pathways of nerve-immunity-blood vessel-barrier, so that the vicious circle of sensitive skin is broken, and the comprehensive effect of quickly relieving symptoms to fundamentally improve the symptoms for a long time is realized.

This invention provides a magnetically and thermally controlled dynamically powered nanomotor neural graft and its preparation method. The neural graft consists of a biodegradable polymer fiber conduit, an internally filled thermosensitive hydrogel, and a nanomotor loaded on the hydrogel. The surface of the nanomotor is loaded with energy molecules such as succinic acid. The nanomotor is prepared using a highly magnetically responsive material and achieves directional guidance under an applied magnetic field. Heat generated under an alternating magnetic field triggers a gel-sol transition in the thermosensitive hydrogel. Energy molecules are continuously released to increase local ATP levels, maintain cellular homeostasis, and support axonal regeneration and synaptic remodeling. This invention combines the advantages of magnetically driven directional navigation and thermosensitive controlled release, overcoming the shortcomings of traditional methods such as insufficient directionality and energy depletion. The graft exhibits good biocompatibility and is biodegradable, can regulate the immune microenvironment of damaged nerves, avoid oxidative stress, and efficiently promote the regeneration and functional recovery of long-segment damaged peripheral nerves.

The application discloses a multifunctional regulation cap type light device based on skull bone marrow-brain function area mapping, and belongs to the technical field of photobiomodulation, transcranial light regulation, neural immune regulation, brain function area positioning and wearable medical device. The device comprises a cap type carrier, a light source array, a function mode selection module, a control unit and a telescopic quasi-direct touch light spot cylinder assembly. After a user selects an emotion, pain, movement, cognition, sleep or visual function irradiation mode through the function mode selection module, the control unit calls corresponding irradiation parameters, irradiation timing and target skull surface projection area, drives the light source unit in the target irradiation partition to work, and keeps the non-target partition light source unit in a closed state. Each light source unit is composed of a telescopic quasi-direct touch light spot cylinder assembly, can adapt to local head curvature through independent telescoping and universal swinging, and can guide light energy to the skull lamella layer in a collimating manner.

The application belongs to the technical field of biological medicine, and particularly relates to the use of SH2 domain-containing protein tyrosine phosphatase 1 (SHP1) in the preparation of a drug for treating chronic pain, in particular to the use of SHP1 in the preparation of a drug for treating chronic pain by regulating the morphology of spinal cord astrocytes, the integrity of the blood-spinal cord barrier and the STAT1-CXCL10-CXCR3 neuroimmune signal axis, and a chronic pain treatment strategy based on the signal axis. The application first discloses that SHP1 in spinal cord astrocytes directly dephosphorylates STAT1, inhibits the transcription of CXCL10 and the subsequent infiltration of T lymphocytes into the spinal cord, and discloses the core analgesic mechanism, thereby establishing SHP1 as a new target for treating chronic pain, and opening up a new way for the precise treatment of clinical chronic pain, especially neuropathic pain.

This invention relates to the field of plant-derived extracellular vesicles, and discloses a method for preparing and applying Hypericum perforatum-derived exosome-like nanovesicles (HPDENs). This invention prepares HPDENs with natural vesicle structures through a specific process, utilizing the natural characteristics of plant-derived vesicles to encapsulate Hypericum perforatum (…). HP The active ingredient, combined with nasal drops, achieves targeted delivery to the brain. It exerts its antidepressant effect by regulating microglia polarization imbalance and reshaping the "neuroimmune-synaptic" axis. This solves the core problems of traditional antidepressants, such as slow onset of action, poor treatment response, and large side effects. It can also improve the core pathological features of depression (neuritis, synaptic plasticity damage) and improve depressive-like behaviors, providing a brand-new option for the clinical treatment of depression.

The invention discloses a medicine for treating neurodevelopment disorder diseases. The invention provides a etiological treatment method capable of crossing a blood brain barrier and directly targeting neural synaptic function repair, stable expression of an exogenous TBL1XR1 gene in a plurality of key brain regions (including hippocampus, prefrontal lobe, basal ganglion and the like) can be realized, and the TBL1XR1 gene has good brain targeting property and tissue affinity, and can be applied to the field of brain diagnosis and treatment. The synaptic disorder in the nervous system and the related nerve development process can be improved, and the composition has good safety in the aspects of system and nerve immunity. The application opens up a new intervention path for the neurodevelopment disorder diseases, especially the neurodevelopment disorder diseases caused by TBL1XR1 gene mutation.

The invention discloses a targeted intestine-brain axis combined medicine which contains mesenchymal stem cells and exosomes derived from the mesenchymal stem cells, and through a stem cell and exosome combined double-targeted treatment strategy, neural immune dysfunction and intestinal flora imbalance are synchronously improved. The combined medicine disclosed by the invention can prevent and / or treat nervous system diseases through various ways of inhibiting prefrontal cortex neuroinflammation, enhancing antioxidation defense of the prefrontal cortex, relieving oxidative damage, repairing a synaptic ultrastructure and adjusting intestinal flora.

The invention discloses a method for constructing a neural immune organoid containing microglial cells, which comprises the following steps: respectively inducing the microglial cells and brain organoid, and co-culturing the brain organoid cultured for about one month and the microglial cells. The addition of PF-127 is beneficial to adhesion of microglial cells to the surface of a brain organ, and the microglial cells can be stably integrated into the brain organ by culturing in a culture medium added with factors M-CSF and IL-3 which are beneficial to survival of the microglial cells, so that a neural immune organ is formed.

The invention discloses a neural immune factor real-time monitoring device, and belongs to the technical field of medical detection. The neural immune factor real-time monitoring device comprises a non-invasive sampling module, a sensing detection module, an intelligent analysis module and a communication feedback module. The invention solves the problem that the existing detection method depends on invasive sampling and cannot realize continuous, stable and synchronous monitoring of multi-target neural immune factors, and realizes non-invasive and automatic sample collection and pretreatment by integrating the hollow microneedle array and the micro-fluidic chip. The sensor array with the automatic calibration unit is used for multi-target synchronous detection, long-term accuracy and anti-interference capability of multi-target synchronous detection are ensured, and in combination with a built-in adaptive calibration algorithm and an intelligent analysis program, accuracy, stability and convenience of long-term monitoring are improved. Effective continuous data support can be provided for dynamic evaluation and early warning of neuroimmune related diseases.

The application provides a neuroimmunological disease immersive virtual patient diagnosis and treatment thinking training system, which comprises a multi-dimensional case holographic modeling module, a diagnosis and treatment thinking anchor point dynamic capturing module, a double-AI collaborative diagnosis and treatment deduction module, an evidence-based feedback and thinking correction module and a diagnosis and treatment capacity quantitative evaluation module which are sequentially connected and constitute a closed loop optimization. The application aims to solve the pain points of "scene static, lack of thinking evaluation, AI scheme deviating from clinic and lack of closed loop optimization" in the existing neuroimmunological disease diagnosis and treatment training.

The present application relates to the technical field of submicron preparation of organic active ingredients, and discloses a nerve immunoregulator targeting microglia cells and a preparation method thereof, which comprises the following steps: mixing a parasite kinase inhibitor and a lipid carrier material in anhydrous ethanol to configure a clear and uniform phase mixed mother liquor, dropping the mixed mother liquor into deionized water, and dispersing and wrapping the mixed mother liquor in a continuous ultrasonic field to form a preparation suspension containing drug lipid microparticles; centrifugally separating the preparation suspension containing drug lipid microparticles to collect precipitates; resuspending the precipitates in a mannitol aqueous solution; and removing water by vacuum freeze-drying. The present application regulates the crystallization kinetics path, inhibits spontaneous aggregation of the raw drug to guarantee the spatial stability of the preparation, improves the selective enrichment degree of central target cells, crosses the barrier along the pathological leakage channel, and releases the active ingredients in situ to calm the neuroimmunological storm.