The invention discloses an expressway intelligent operation system through coordination of automobiles and pathways and a using method. The system comprises two pathways which are arranged at the ascending side and the descending side of an expressway in the same direction, and at least one veering rail is connected between the pathways at the two sides, the veering rail is communicated with the pathways, and the system further comprises transportation carts which can load automobiles and run on the pathways, control panels, control chips, locating devices, and a central running system, wherein the control panels, control chips, and locating devices are arranged on the corresponding transportation carts, and the central running system is used for regulating and controlling all the transportation carts. Through the method of combining the automobiles with the pathways, the purpose of conducting door-to-door transportation can be achieved, and the transportation time is saved; the central running system is utilized to intelligently regulate and control all the transportation carts, and thus unmanned driving is achieved; after getting rest in one transportation cart and getting off the transportation cart, a driver keeps a better mental state to drive on a common expressway, and thus an car accident caused by fatigue driving is avoided; it is guaranteed that green-hand drivers can be helped to safely drive on the expressway.